Characterizing phage-host interactions in a simplified human intestinal barrier model

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dc.contributor.authorNúñez-Sánchez, María A.en
dc.contributor.authorColom, Joanen
dc.contributor.authorWalsh, Laurenen
dc.contributor.authorButtimer, Colinen
dc.contributor.authorBolocan, Andrei Sorinen
dc.contributor.authorPang, Roryen
dc.contributor.authorGahan, Cormac G.en
dc.contributor.authorHill, Colinen
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderJohnson & Johnson Innovative Medicineen
dc.contributor.funderEuropean Commissionen
dc.contributor.funderIrish Research Councilen
dc.date.accessioned2024-03-27T16:43:59Z
dc.date.available2024-03-27T16:43:59Z
dc.date.issued2020en
dc.description.abstractAn intestinal epithelium model able to produce mucus was developed to provide an environment suitable for testing the therapeutic activity of gut bacteriophages. We show that Enterococcus faecalis adheres more effectively in the presence of mucus, can invade the intestinal epithelia and is able to translocate after damaging tight junctions. Furthermore, Enterococcus phage vB_EfaM_A2 (a member of Herelleviridae that possesses virion associated immunoglobin domains) was found to translocate through the epithelium in the presence and absence of its host bacteria. Phage A2 protected eukaryotic cells by reducing mortality and maintaining the structure of the cell layer structure. We suggest the mammalian cell model utilized within this study as an adaptable in vitro model that can be employed to enable a better understanding of phage–bacteria interactions and the protective impact of phage therapy relating to the intestinal epithelium.en
dc.description.sponsorshipIrish Research Council (Government of Ireland Postdoctoral Fellowship 2019, Project ID GOIPD/2019/1097)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid1374en
dc.identifier.citationNúñez-Sánchez, M.A., Colom, J., Walsh, L., Buttimer, C., Bolocan, A.S., Pang, R., Gahan, C.G.M. and Hill, C. (2020) ‘Characterizing phage-host interactions in a simplified human intestinal barrier model’, Microorganisms, 8(9), 1374 (20pp). doi: 10.3390/microorganisms8091374en
dc.identifier.doi10.3390/microorganisms8091374en
dc.identifier.eissn2076-2607en
dc.identifier.endpage20en
dc.identifier.issued9en
dc.identifier.journaltitleMicroorganismsen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/15715
dc.identifier.volume8en
dc.language.isoenen
dc.publisherMDPIen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI ERC Development Programme/15/ERCD/3189/IE/GUTPHAGE: bacteriophage as agents to manipulate the microbiota for human health/en
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Spokes Programme/14/SP APC/B3032/IE/Gut Phageomics - Phage as diagnostics and/or therapeutics in IBD/en
dc.relation.projectinfo:eu-repo/grantAgreement/EC/H2020::MSCA-IF-EF-ST/796838/EU/Bile acids and epithelial stem cells in intestinal development/BESIDEen
dc.rights© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectEnterococcus faecalisen
dc.subjectBacteriophageen
dc.subjectHerelleviridaeen
dc.subjectIntestinal modelen
dc.subjectPhage therapyen
dc.subjectIBDen
dc.titleCharacterizing phage-host interactions in a simplified human intestinal barrier modelen
dc.typeArticle (peer-reviewed)en
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