Physiological bioactivity of a postbiotic consisting of heat-treated lactobacilli on mouse small intestine

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dc.contributor.authorUhlig, Friederike
dc.contributor.authorWarda, Alicja K.
dc.contributor.authorHueston, Cara M.
dc.contributor.authorDraper, Lorraine A.
dc.contributor.authorChauvière, Gilles
dc.contributor.authorEckhardt, Erik
dc.contributor.authorHill, Colin
dc.contributor.authorHyland, Niall P.
dc.date.accessioned2023-09-26T15:33:45Z
dc.date.available2023-09-26T09:04:11Zen
dc.date.available2023-09-26T15:33:45Z
dc.date.issued2023-08-16
dc.date.updated2023-09-26T08:04:16Zen
dc.description.abstractLactobacillus LB is a postbiotic generated following fermentation by Limosilactobacillus fermentum and Lactobacillus delbrueckii. Lactobacillus LB alleviates acute diarrhoea and ameliorates the symptoms of irritable bowel syndrome. Here, we investigated whether modulation of intestinal ion transport and motility contributes to these beneficial effects and whether the postbiotic produced with both strains contributes to a unique biophysiological profile. In Ussing chamber studies, low lactose-Lactobacillus LB (LL-LB) significantly increased baseline short-circuit current, and this was partially mediated by sodium-D-glucose transporter 1. In organ baths, LL-LB significantly decreased ileal tone and increased carbachol-induced contractility. Relative to LL-LB, preparations produced using a single strain fermentate generated from L. fermentum significantly increased baseline short-circuit current and inhibited carbachol-induced contractility. Our data demonstrate a unique biophysiological profile for the dual strain postbiotic and support a direct and immediate effect of LL-LB on host physiology ex vivo which could contribute to the clinical efficacy of Lactobacillus LB.en
dc.description.statusPeer revieweden
dc.description.versionPublished Version
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid105730
dc.identifier.citationUhlig, F., Warda, A.K., Hueston, C.M., Draper, L.A., Chauvière, G., Eckhardt, E., Hill, C. and Hyland, N.P. (2023) ‘Physiological bioactivity of a postbiotic consisting of heat-treated lactobacilli on mouse small intestine’, Journal of Functional Foods, 108, 105730 (9 pp). https://doi.org/10.1016/j.jff.2023.105730
dc.identifier.doi10.1016/j.jff.2023.105730en
dc.identifier.endpage9
dc.identifier.issn1756-4646
dc.identifier.journaltitleJournal of Functional Foods
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/10468/15028
dc.language.isoenen
dc.publisherElsevier
dc.relation.ispartofJournal of Functional Foods
dc.relation.urihttps://doi.org/10.1016/j.jff.2023.105730
dc.rights© 2023 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectLactobacilli
dc.subjectFermentate
dc.subjectMotility
dc.subjectPostbiotic
dc.subjectShort-circuit current
dc.subjectUssing chamber
dc.titlePhysiological bioactivity of a postbiotic consisting of heat-treated lactobacilli on mouse small intestineen
dc.typeArticle (peer-reviewed)
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