Bifidobacterium animalis AHC7 protects against pathogen-induced NF-ҝB activation in vivo

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dc.contributor.authorO'Mahony, David
dc.contributor.authorMurphy, Sharon
dc.contributor.authorBoileau, Thomas
dc.contributor.authorPark, JeanSoon
dc.contributor.authorO'Brien, Frances
dc.contributor.authorGroeger, David S.
dc.contributor.authorKonieczna, Patrycja
dc.contributor.authorZiegler, Mario
dc.contributor.authorScully, Paul
dc.contributor.authorShanahan, Fergus
dc.contributor.authorKiely, Barry
dc.contributor.authorO'Mahony, Liam
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderHealth Research Boarden
dc.contributor.funderHigher Education Authorityen
dc.contributor.funderSchweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschungen
dc.contributor.funderAlimentary Healthen
dc.contributor.funderProcter and Gambleen
dc.date.accessioned2012-11-12T13:34:00Z
dc.date.available2012-11-12T13:34:00Z
dc.date.copyright2010
dc.date.issued2010-12-22
dc.description.abstractBackground: Bifidobacteria and lactobacilli are among the early and important colonizers of the gastrointestinal tract and are generally considered to be part of a normal, healthy microbiota. It is believed that specific strains within the microbiota can influence host immune-reactivity and may play a role in protection from infection and aberrant inflammatory activity. One such strain, Bifidobacterium animalis AHC7, has been previously shown to protect against Salmonella typhimurium infection in mice and helps resolve acute idiopathic diarrhea in dogs. The aim of this study was to investigate the potential molecular and cellular mechanisms underpinning the Bifidobacterium animalis AHC7 protective effect. Results: Following 4 hours of infection with Salmonella typhimurium, NF-ҝB activation was significantly elevated in vivo in placebo and Enterococcus faecium-fed animals while Bifidobacterium animalis AHC7 consumption significantly attenuated the NF- B response. In vitro anti-CD3/CD28 stimulated Peyer’s patch cells secreted significantly less TNF-a and IFN-g following Bifidobacterium animalis AHC7 consumption. Stimulated cells released more IL-12p70 but this difference did not reach statistical significance. No alteration in mucosal IL-6, IL-10 or MCP-1 levels were observed. No statistically significant change in the cytokine profile of mesenteric lymph node cells was noted. In vitro, Bifidobacterium animalis AHC7 was bound by dendritic cells and induced secretion of both IL-10 and IL-12p70. In addition, co-culture of CD4+ T cells with Bifidobacterium animalis AHC7-stimulated dendritic cells resulted in a significant increase in CD25+Foxp3+ T cell numbers. Conclusion: Bifidobacterium animalis AHC7 exerts an anti-inflammatory effect via the attenuation of proinflammatory transcription factor activation in response to an infectious insult associated with modulation of proinflammatory cytokine production within the mucosa. The cellular mechanism underpinning Bifidobacterium animalis AHC7 mediated attenuation of NF- B activation may include recognition of the bacterium by dendritic cells and induction of CD25+Foxp3+ T cells.en
dc.description.sponsorshipScience Foundation Ireland (SFI-CSET)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationO'Mahony D, Murphy S, Boileau T, Park JS, O'Brien F, Groeger D, Konieczna P, Ziegler M, Scully P, Shanahan F, Kiely B, O'Mahony L. Bifidobacterium animalis AHC7 protects against pathogen-induced NF-κB activation in vivo. (2010) BMC Immunology. 11:63. doi: 10.1186/1471-2172-11-63en
dc.identifier.doi10.1186/1471-2172-11-63
dc.identifier.issn1471-2172
dc.identifier.issued11en
dc.identifier.journaltitleBMC Immunologyen
dc.identifier.startpage63en
dc.identifier.urihttps://hdl.handle.net/10468/758
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urihttp://www.biomedcentral.com/1471-2172/11/63
dc.rights© 2010 O'Mahony et al; licensee BioMed Central Ltd.en
dc.rights.urihttp://creativecommons.org/licenses/by/2.0en
dc.subjectBifidobacterium animalis AHC7en
dc.subjectSalmonella typhimuriumen
dc.subjectNF-ҝBen
dc.subjectIdiopathic Diarrheaen
dc.subjectin vivoen
dc.subjectAnti-inflammatory Effecten
dc.titleBifidobacterium animalis AHC7 protects against pathogen-induced NF-ҝB activation in vivoen
dc.typeArticle (peer-reviewed)en
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