Conjugated linoleic acid (CLA)-enriched milk fat inhibits growth and modulates CLA-responsive biomarkers in MCF-7 and SW480 human cancer cell lines

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dc.contributor.authorMiller, Aine
dc.contributor.authorStanton, Catherine
dc.contributor.authorMurphy, John
dc.contributor.authorDevery, Rosaleen
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2013-04-30T13:37:12Z
dc.date.available2013-04-30T13:37:12Z
dc.date.copyright2003
dc.date.issued2003-07
dc.description.abstractMilk enriched in conjugated linoleic acid (CLA) was obtained from cows on pasture supplemented with full-fat rapeseeds (FFR; 2·26g cis 9, trans 11 (c9, t11)-CLA/100g fatty acid methyl esters) and full-fat soyabeans (1·83g c9, t11-CLA/100g fatty acid methyl esters). A control milk fat (1·69g c9, t11-CLA/100g fatty acid methyl esters) was obtained from cows fed on pasture only. The present study assessed the potency of the CLA-enriched milk fats to modulate biomarkers that had previously been observed to respond to c9, t11-CLA in the MCF-7 and SW480 cell lines. Cell numbers decreased (P<0·05) by up to 61 and 58% following the incubation of MCF-7 and SW480 cells, respectively, for 4d with milk fats (yielding CLA concentrations between 60·2 and 80·6μM). The FFR milk fat, containing the highest CLA content, increased (P<0·05) [14C]arachidonic acid (AA) uptake into the monoacylglycerol fraction of MCF-7 and SW480 cells while it decreased (P<0·05) uptake into the phospholipid fraction of the latter. This milk fat also decreased (P<0·05) [14C]AA conversion to prostaglandin (PG) E2 while increasing conversion to PGF2α in both cell lines. All milk-fat samples increased (P<0·05) lipid peroxidation as measured by 8-epi-PGF2α in both cell lines. In SW480 cells the milk-fat samples decreased (P<0·05) bcl-2 and cytosolic glutathione levels while increasing (P<0·05) membrane-associated annexin V levels. All milk-fat samples decreased (P<0·05) the expression of ras in SW480 cells. These data suggest that milk-fat CLA was effective at modulating synthetic CLA-responsive biomarkers.en
dc.description.sponsorshipScience Foundation Ireland (SFI-CSET)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationMiller, A., Stanton, C., Murphy, J., and Devery, R. (2003). Conjugated linoleic acid (CLA)-enriched milk fat inhibits growth and modulates CLA-responsive biomarkers in MCF-7 and SW480 human cancer cell lines. British Journal of Nutrition, 90, 5: 877-885. doi: 10.1079/BJN2003978en
dc.identifier.doi10.1079/BJN2003978
dc.identifier.endpage885en
dc.identifier.issued5en
dc.identifier.journaltitleBritish Journal of Nutritionen
dc.identifier.startpage877en
dc.identifier.urihttps://hdl.handle.net/10468/1103
dc.identifier.volume90en
dc.language.isoenen
dc.publisherCambridge University Pressen
dc.relation.urihttp://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=910872&fulltextType=RA&fileId=S0007114503002009
dc.rights© The Nutrition Society 2003en
dc.subjectMilk faten
dc.subjectConjugated linoleic aciden
dc.subjectEicosanoidsen
dc.subjectApoptosisen
dc.subject.lcshMicrobiology.en
dc.titleConjugated linoleic acid (CLA)-enriched milk fat inhibits growth and modulates CLA-responsive biomarkers in MCF-7 and SW480 human cancer cell linesen
dc.typeArticle (peer-reviewed)en
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