- ItemNon-invasive multimodal spectroscopic diagnosis for early-stage oral cancer(Optica Publishing Group, 2023) Maryam, Siddra; Ghauri, Daniyal; Sekar, Sanathana Konugolu Venkata; Fahy, Edward; Saito Nogueira, Marcelo; Lu, Huihui; Beffara, Flavien; Humbert, Georges; Burke, Ray; Feeley, Linda; Sheahan, Patrick; Ni Riordain, Richeal; Andersson-Engels, Stefan; Wei Kho, Kiang; Gautam, Rekha; 2023This study aims to develop a multimodal scheme for diagnosing oral cancer non-invasively in its early stages and to assess the performance of an integrated diagnostic platform comprising of Raman and diffuse reflectance spectroscopy systems.
- ItemMulti-configuration Raman spectrometer for early stage diagnosis of oral cancer(Society of Photo-Optical Instrumentation Engineers (SPIE), 2022-03-02) Maryam, Siddra; Saito Nogueira, Marcelo; Krishna Moorthy, Shree; Sekar, Sanathana Konugolu Venkata ; Lu, Huihui; Gautam, Rekha; Burke, Ray; Andersson-Engels, Stefan; Ni Riordain, Richeal; Sheahan, Patrick; Huang, Zhiwei; Science Foundation IrelandOral Squamous Cell carcinoma (OSCC) is one of the most common and aggressive oral malignancies. Despite all significant advances in medicine, five-year survival rate is still 40%-60%. Diagnosis in early stages is critical as it can improve the survival rate and the quality of life after treatment. This study aims to develop a strategy for diagnosing oral cancer non-invasively in the early stages and to provide better surgical guidance by differentiating healthy and tumor tissues by using Raman spectroscopy. For this purpose, a multimodal Raman system is developed to detect oral cancer biomarkers in patient’s saliva specimen and to study different tissue types in oral cavity with Raman spectroscopy. The developed system is quite compact, easy to use and portable. It can be easily modified for in vivo and ex vivo analysis and can work in both reflection and transmission mode in case of ex vivo measurements. This paper compares the surface enhancement and background spectra from different plasmonic nanoparticles. Lastly, bovine serum albumin (BSA) and uric acid were used as model analytes to at physiologically relevant concentrations to test the performance of the system.
- ItemMultimodal optical spectroscopic approach to design a complete protocol for early diagnosis of oral cancer(Society of Photo-Optical Instrumentation Engineers (SPIE), 2023-03-06) Maryam, Siddra; Ghauri, M. Daniyal; Fahy, Edward; Sekar, Sanathana Konugolu Venkata; Lu, Huihui; Russo, Alida; Saito Nogueira, Marcelo; Burke, Ray; Feeley, Linda; Sheahen, Patrick; Ni Riordain, Richeal; Andersson-Engels, Stefan; Kho, Kiang Wei; Gautam, Rekha; Boudoux, Caroline; Tunnell, James W.; Science Foundation IrelandOral cancer is one of the most malignant cancers in the world. Early-stage diagnosis of oral cancer is complex process due to the multifocal unspecific development of non-malignant lesions into cancer and impossibility to take biopsy of every lesion. The aim of this study is to develop a screening method for oral cancer diagnosis at early stages using surface enhanced Raman spectroscopy (SERS) and validate the performance of a multimodal system including Raman spectroscopic (RS) and diffuse reflectance spectroscopy (DRS) oral cancer diagnosis and accurate margin detection. The study will involve the identification and integration of spectral biomarkers involved in the carcinogenesis process from different modalities. Each modality SERS, RS and DRS is calibrated and standardized individually. Patients suffering from oral squamous cell carcinoma and other malignant diseases going through biopsy or histopathological examination are enrolled in this study. Ex vivo study involves the SERS analysis of saliva specimen and in vivo analysis will involve measurements on various tissue types, including malignant tissue and healthy contralateral site to evaluate the reproducibility and signal-to-noise ratio using fiber-optic probes for Raman and DRS systems. Feature selection methods and further machine learning tools will be used to discriminate between healthy, benign and cancer lesions based on spectral information and to identify important biomarkers. After data collection, clinician will perform a normal biopsy procedure and histopathological analysis, which will serve as gold standard to determine the sensitivity and specificity of the spectroscopy techniques.
- ItemWorld Workshop on Oral Medicine VIII: Development of a core outcome set for oral lichen planus: The patient perspective(Elsevier Inc., 2023-03-01) Diniz-Freitas, Márcio; López-Pintor, Rosa María; Bissonnette, Caroline; Dan, Hongxia; Kuduva Ramesh, Shilpa Shree; Valdéz, J. Amadeo; Brennan, Michael T.; Burkhart, Nancy W.; Farag, Arwa; Greenberg, Martin S.; Hong, Catherine; Setterfield, Jane F.; Woo, Sook-Bin; Sollecito, Thomas P.; Byrne, Harriet; Robledo-Sierra, Jairo; Taylor, Jennifer; Ni Riordain, RichealObjective: This study aimed to explore the lived experience of patients with oral lichen planus (OLP) and investigate what treatment-related outcomes are the most important to them and should be included in a core outcome set (COS) for OLP. Study Design: A qualitative study involving focus group work with 10 participants was conducted. Interviews with each focus group were held twice: session 1 explored the lived experience of patients with OLP, and session 2 allowed patients to review a summary of the outcome domains used in the OLP literature to date. The discussions were recorded, transcribed verbatim, and analyzed using framework analysis. Results: In session 1, 4 themes and 8 sub-themes emerged from the data analysis. An additional outcome, ‘knowledge of family and friends,’ was suggested in session 2. Conclusions: We have gained valuable insight into the lived experience of patients with OLP via this qualitative study. To our knowledge, this study is the first to explore the patient perspective on what should be measured in clinical trials on OLP, highlighting an important additional suggested outcome. This additional outcome will be voted upon in a consensus process to determine a minimum COS for OLP.
- ItemStudent professionalism: calibration of dental assessors(2019-10) Charrette, David; Hayes, Martina; Roberts, Anthony; University College CorkCork Dental School & Hospital (CUDSH) uses two reporting methods to longitudinally assess Dental student (BDS) professionalism; Clinical Grading (CG) or Traffic Light Reporting (TLR). These evaluations occur in clinical and non-clinical environments by Clinical Staff (CS) and Non-Clinical Staff (NCS). The objective of the study was to develop a series of professionalism scenarios and compare the perceptions of BDS and staff (CS & NCS) of each scenario and preferred reporting scheme. Staff evaluation formed part of a calibration event. 13 professionalism-based scenarios were administered to Final Year BDS students (n=30) using Google Forms. During a staff calibration seminar the same scenarios were presented to CS (n=12) and NCS (n=36). Each scenario varied in its seriousness, environment and staff role. The use of the TLR for BDS and CS was 57.4% and 60% respectively but was used by NCS in 74.3% of scenarios. NCS awarded Red cards more frequently (46.7%) than BDS (29.0%) or CS (31.6%) and were least likely to take no further action. There was little variation in the award of green cards…There was a high level of agreement between BDS, CS and NCS in the award and reporting of professionalism. NCS were more likely to use TLR and for poor professionalism were most likely to award Red Cards for serious events. The study ensured BDS and staff familiarity with common professionalism scenarios across the School and an opportunity for calibration of staff in the grading and reporting of professionalism standards.