- ItemBifidobacterium transformation(Springer Science+Business Media, LLC, 2021-03-03) Hoedt, Emily C.; Bongers, Roger S.; Bottacini, Francesca; Knol, Jan; MacSharry, John; van Sinderen, Douwe; van Sinderen, Douwe; Ventura, Marco; Nutricia Research Foundation; Science Foundation IrelandThe protocol presented in this chapter describes a generic method for electrotransformation of Bifidobacterium spp., outlining a technique that is ideal for conferring selective properties onto strains as well as allowing the user to introduce or knock out/in selected genes for phenotypic characterization purposes. We have generalized on the plasmid chosen for transformation and antibiotic selection marker, but the protocol is versatile in this respect and we are able to achieve transformation efficiencies up to 107 transformants/μg of DNA.
- ItemInterventional Radiology in oncology.(Springer Science+Business Media, 2008-01) O'Connor, Owen J.; Buckley, J. M.; Maher, Michael M.; Blake, Michael A.; Kalra, Mannudeep K.Since its development as a sub-speciality within radiology, Interventional Radiology (IR) has played an increasingly important role in caring for the patient with cancer. This role begins with initial diagnosis of cancer and involvement now extends into minimally invasive treatment of malignancy alone or in combination with other treatment modalities. IR has established a very important role in the management of complications incurred during many oncological treatments. This chapter provides an updated overview of the scope of IR in the management of the oncology patient.