Demonstration of three-dimensional optical imaging using a confocal microscope based on a liquid-crystal electronic lens

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dc.contributor.author Riza, Nabeel A.
dc.contributor.author Sheikh, Mumtaz A.
dc.contributor.author Webb-Wood, Grady
dc.contributor.author Kik, Pieter G.
dc.date.accessioned 2020-05-25T14:45:54Z
dc.date.available 2020-05-25T14:45:54Z
dc.date.issued 2008-06-01
dc.identifier.citation Riza, N. A., Sheikh, M. A., Webb-Wood, G. and Kik, P. K. (2008) ‘Demonstration of three-dimensional optical imaging using a confocal microscope based on a liquid-crystal electronic lens’, Optical Engineering, 47(6), 063201 (9 pp). doi: 10.1117/1.2944135 en
dc.identifier.volume 47 en
dc.identifier.issued 8 en
dc.identifier.startpage 1 en
dc.identifier.endpage 9 en
dc.identifier.issn 0091-3286
dc.identifier.issn 1560-2303
dc.identifier.uri http://hdl.handle.net/10468/10043
dc.identifier.doi 10.1117/1.2944135 en
dc.description.abstract Three-dimensional (3-D) imaging is demonstrated using an electronically controlled liquid crystal (LC) optical lens to accomplish a no-moving-parts depth-section scanning in a modified commercial 3-D confocal microscope. Specifically, 3-D views of a standard CDC blood vessel (enclosed in a glass slide) have been obtained using the modified confocal microscope operating at the red 633-nm laser wavelength. The image sizes over a 25-μm axial scan depth were 50×50 μm and 80×80 μm, using 60× and 20× micro-objectives, respectively. The transverse motion step was 0.1 μm for the 60× data and 0.2 μm for the 20× data. As a first-step comparison, image processing of the standard and LC electronic-lens microscope images indicates correlation values between 0.81 and 0.91. The proposed microscopy system within aberration limits has the potential to eliminate the mechanical forces due to sample or objective motion that can distort the original sample structure and lead to imaging errors. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Society of Photo-optical Instrumentation Engineers (SPIE) en
dc.rights © 2008 Society of Photo-Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. en
dc.subject Liquid crystal en
dc.subject Optical imaging en
dc.subject Microscopy en
dc.subject Microscopes en
dc.subject Confocal microscopy en
dc.subject 3D image processing en
dc.subject Imaging systems en
dc.subject Monochromatic aberrations en
dc.subject Arteries en
dc.title Demonstration of three-dimensional optical imaging using a confocal microscope based on a liquid-crystal electronic lens en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Nabeel Riza, School of Engineering, University College Cork, Cork, Ireland. +353-21-490-3000 Email: n.riza@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Optical Engineering en
dc.internal.IRISemailaddress n.riza@ucc.ie en
dc.identifier.articleid 063201 en


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