Demonstration of three-dimensional optical imaging using a confocal microscope based on a liquid-crystal electronic lens
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Published version
Date
2008-06-01
Authors
Riza, Nabeel A.
Sheikh, Mumtaz A.
Webb-Wood, Grady
Kik, Pieter G.
Journal Title
Journal ISSN
Volume Title
Publisher
Society of Photo-optical Instrumentation Engineers (SPIE)
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Abstract
Three-dimensional (3-D) imaging is demonstrated using an electronically controlled liquid crystal (LC) optical lens to accomplish a no-moving-parts depth-section scanning in a modified commercial 3-D confocal microscope. Specifically, 3-D views of a standard CDC blood vessel (enclosed in a glass slide) have been obtained using the modified confocal microscope operating at the red 633-nm laser wavelength. The image sizes over a 25-μm axial scan depth were 50×50 μm and 80×80 μm, using 60× and 20× micro-objectives, respectively. The transverse motion step was 0.1 μm for the 60× data and 0.2 μm for the 20× data. As a first-step comparison, image processing of the standard and LC electronic-lens microscope images indicates correlation values between 0.81 and 0.91. The proposed microscopy system within aberration limits has the potential to eliminate the mechanical forces due to sample or objective motion that can distort the original sample structure and lead to imaging errors.
Description
Keywords
Liquid crystal , Optical imaging , Microscopy , Microscopes , Confocal microscopy , 3D image processing , Imaging systems , Monochromatic aberrations , Arteries
Citation
Riza, N. A., Sheikh, M. A., Webb-Wood, G. and Kik, P. K. (2008) ‘Demonstration of three-dimensional optical imaging using a confocal microscope based on a liquid-crystal electronic lens’, Optical Engineering, 47(6), 063201 (9 pp). doi: 10.1117/1.2944135
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© 2008 Society of Photo-Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.