dc.contributor.author |
Burgess, Catherine M. |
|
dc.contributor.author |
Smid, Eddy J. |
|
dc.contributor.author |
Rutten, Ger |
|
dc.contributor.author |
van Sinderen, Douwe |
|
dc.date.accessioned |
2013-04-08T15:14:53Z |
|
dc.date.available |
2013-04-08T15:14:53Z |
|
dc.date.copyright |
2006 |
|
dc.date.issued |
2006-07 |
|
dc.identifier.citation |
Burgess CM, Smid EJ, Rutten G, van Sinderen D. (2006) A general method for selection of riboflavin-overproducing food grade micro-organisms. Microbial Cell Factories, 5: 24. doi: 10.1186/1475-2859-5-24 |
en |
dc.identifier.volume |
5 |
en |
dc.identifier.issued |
24 |
en |
dc.identifier.uri |
http://hdl.handle.net/10468/1029 |
|
dc.identifier.doi |
10.1186/1475-2859-5-24 |
|
dc.description.abstract |
Background: This study describes a strategy to select and isolate spontaneous riboflavin overproducing
strains of Lactobacillus (Lb.) plantarum, Leuconostoc (Lc.) mesenteroides and
Propionibacterium (P.) freudenreichii.
Results: The toxic riboflavin analogue roseoflavin was used to isolate natural riboflavinoverproducing
variants of the food grade micro-organisms Lb. plantarum, Lc. mesenteroides and P.
freudenreichii strains. The method was successfully employed for strains of all three species. The
mutation(s) responsible for the observed overproduction of riboflavin were identified for isolates
of two species.
Conclusion: Selection for spontaneous roseoflavin-resistant mutants was found to be a reliable
method to obtain natural riboflavin-overproducing strains of a number of species commonly used
in the food industry. This study presents a convenient method for deriving riboflavin-overproducing
strains of bacterial starter cultures, which are currently used in the food industry, by a nonrecombinant
methodology. Use of such starter strains can be exploited to increase the vitamin
content in certain food products. |
en |
dc.description.sponsorship |
Science Foundation Ireland (SFI-CSET); |
en |
dc.format.mimetype |
application/pdf |
en |
dc.language.iso |
en |
en |
dc.publisher |
BioMed Central |
en |
dc.relation.uri |
http://www.microbialcellfactories.com/content/5/1/24 |
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dc.rights |
© 2006 Burgess et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
en |
dc.rights.uri |
http://creativecommons.org/licenses/by/2.0 |
en |
dc.subject |
Riboflavin |
en |
dc.subject |
Roseoflavin |
en |
dc.subject |
Lactobacillus plantarum |
en |
dc.subject |
Leuconostoc mesenteroides |
en |
dc.subject |
Propionibacterium freudenreichii |
en |
dc.title |
A general method for selection of riboflavin-overproducing food grade micro-organisms |
en |
dc.type |
Article (peer-reviewed) |
en |
dc.internal.authorurl |
http://publish.ucc.ie/researchprofiles/D010/dvansinderen |
en |
dc.internal.authorcontactother |
Douwe van Sinderen, Alimentary Pharmabiotic Centre, University College Cork, Cork. E-mail: d.vansinderen@ucc.ie |
en |
dc.internal.availability |
Full text available |
en |
dc.description.version |
Published Version |
en |
dc.contributor.funder |
Science Foundation Ireland |
en |
dc.description.status |
Peer reviewed |
en |
dc.identifier.journaltitle |
Microbial Cell Factories |
en |
dc.internal.copyrightchecked |
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
en |
dc.internal.IRISemailaddress |
d.vansinderen@ucc.ie |
en |