Inhibitory activity of Lactobacillus plantarum LMG P-26358 against Listeria innocua when used as an adjunct starter in the manufacture of cheese
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Date
2011-08
Authors
Mills, S.
Serrano, L. M.
O'Connor, Paula M.
Griffin, Carmel
Schaad, Gwenda
Bruining, Chris
Hill, Colin
Ross, R. Paul
Meijer, Wilco C.
Journal Title
Journal ISSN
Volume Title
Publisher
BioMed Central
Published Version
Abstract
Lactobacillus plantarum LMG P-26358 isolated from a soft French artisanal cheese produces a potent class IIa bacteriocin with 100% homology to plantaricin 423 and bacteriocidal activity against Listeria innocua and Listeria monocytogenes. The bacteriocin was found to be highly stable at temperatures as high as 100°C and pH ranges from 1-10. While this relatively narrow spectrum bacteriocin also exhibited antimicrobial activity against species of enterococci, it did not inhibit dairy starters including lactococci and lactobacilli when tested by well diffusion assay (WDA). In order to test the suitability of Lb. plantarum LMG P-26358 as an anti-listerial adjunct with nisin-producing lactococci, laboratory-scale cheeses were manufactured. Results indicated that combining Lb. plantarum LMG P-26358 (at 108 colony forming units (cfu)/ml) with a nisin producer is an effective strategy to eliminate the biological indicator strain, L. innocua. Moreover, industrial-scale cheeses also demonstrated that Lb. plantarum LMG P-26358 was much more effective than the nisin producer alone for protection against the indicator. MALDI-TOF mass spectrometry confirmed the presence of plantaricin 423 and nisin in the appropriate cheeses over an 18 week ripening period. A spray-dried fermentate of Lb. plantarum LMG P-26358 also demonstrated potent anti-listerial activity in vitro using L. innocua. Overall, the results suggest that Lb. plantarum LMG P-26358 is a suitable adjunct for use with nisin-producing cultures to improve the safety and quality of dairy products
Description
Keywords
Lactobacillus plantarum , French artisanal cheese , Bacteriocin , Plantaricin 423 , Listeria innocua , Listeria monocytogenes , Nisin-producing culture
Citation
Mills, S., Serrano, L.M., Griffin, C., O. Connor, P.M, Schaad G., Bruining C., Hill, C., Ross, R.P., Meijer, W.C. (2011) Inhibitory Activity of Lactobacillus plantarum LMG P-26358 against Listeria innocua when used as an Adjunct Starter in the Manufacture of Cheese. Microbial Cell Factories 10(Suppl 1):S7