Exploiting the diverse microbial ecology of marine sponges

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dc.contributor.advisor Dobson, Alan D. W. en
dc.contributor.advisor Morrissey, John P. en
dc.contributor.author Jackson, Stephen A.
dc.date.accessioned 2013-04-09T11:23:12Z
dc.date.available 2016-04-09T04:00:05Z
dc.date.issued 2013
dc.date.submitted 2013
dc.identifier.citation Jackson, S. A. 2013. Exploiting the diverse microbial ecology of marine sponges. PhD Thesis, University College Cork. en
dc.identifier.endpage 290
dc.identifier.uri http://hdl.handle.net/10468/1037
dc.description.abstract Marine sponges (phylum Porifera) are the oldest extant metazoan animals on earth and host large populations of symbiotic microbes: Bacteria, Archaea and unicellular Eukaryota. Those microbes play ecological functions which are essential to the health of the host including carbon, nitrogen and sulfur cycling as well as host defence through the production of bioactive secondary metabolites which protect against infection and predation. The diversity of sponge-associated microbes is remarkable with thousands of OTUs reported from individual sponge species. Amongst those populations are sponge-specific microbes which may be specific to sponges or specific to sponge species. While marine natural product discovery concerns many animal phyla, Porifera account for the largest proportion of novel compounds. Evidence suggests that many of these compounds are the products of symbiotic microbes. Descriptions of sponge-associated microbial community structures have been advanced by the development of next-generation sequencing technologies while the discovery and exploitation of sponge derived bioactive compounds has increased due to developments in sequence-based and function-based metagenomics. Here, we use pyrosequencing to describe the bacterial communities associated with two shallow, temperate water sponges (Raspailia ramosa and Stelligera stuposa) from Irish coastal waters and to describe the bacterial and archaeal communities of a single sponge species (Inflatella pellicula) from two different depths in deep waters in the Atlantic Ocean, including at a depth of 2900m, a depth far greater than that of any previous sequence-based sponge-microbe investigation. We identified diverse microbial communities in all sponges and the presence of sponge-specific taxa recruiting to previously described and novel spongespecific clusters. We also identified archaeal communities which dominated sponge-microbe communities. We demonstrate that sponge-associated microbial communities differ from seawater communities indicating host selection processes. We used sequence-based metagenomic techniques to identify genes of potential industrial and pharmacological interest in the metagenomes of various sponge species and functionbased metagenomic screening in an attempt to identify lipolytic and antibacterial activities from metagenomic clones from the metagenome of the marine sponge Stelletta normani. In addition we have cultured diverse bacterial species from sponge tissues, many of which display antimicrobial activities against clinically relevant bacterial and yeast test strains. Other isolates represent novel species in the genus Maribacter and require emendments to the description of that genus. en
dc.description.sponsorship Marine Institute (Beaufort Marine Research Award) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher University College Cork en
dc.rights © 2013, Stephen A. Jackson en
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/ en
dc.subject Marine sponges en
dc.subject Porifera en
dc.subject Metazoa en
dc.subject Symbiotic microbes en
dc.subject Sponge derived bioactive compounds en
dc.subject Metagenomics en
dc.subject Pyrosequencing en
dc.subject Raspailia ramosa en
dc.subject Stelligera stuposa en
dc.subject Inflatella pellicula en
dc.subject Stelletta normani en
dc.subject Maribacter en
dc.subject.lcsh Sponges en
dc.title Exploiting the diverse microbial ecology of marine sponges en
dc.type Doctoral thesis en
dc.type.qualificationlevel Doctoral en
dc.type.qualificationname PhD (Science) en
dc.internal.availability Full text available en
dc.description.version Accepted Version
dc.contributor.funder Marine Institute en
dc.description.status Not peer reviewed en
dc.internal.school Microbiology en
dc.check.reason This thesis is due for publication or the author is actively seeking to publish this material en
dc.check.opt-out Not applicable en
dc.thesis.opt-out false *
dc.check.entireThesis Entire Thesis Restricted
dc.check.embargoformat Both hard copy thesis and e-thesis en
ucc.workflow.supervisor a.dobson@ucc.ie *
ucc.workflow.supervisor cora@ucc.ie *
ucc.workflow.supervisor cora@ucc.ie *

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© 2013, Stephen A. Jackson Except where otherwise noted, this item's license is described as © 2013, Stephen A. Jackson
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