Access to this article is restricted until 12 months after publication by request of the publisher.. Restriction lift date: 2021-02-03
The effect of sphingosine‐1‐phosphate on the endothelial glycocalyx during ischemia‐reperfusion injury in the isolated rat heart
Loading...
Files
Accepted Version
Date
2020-02-03
Authors
Araibi, Hala
van der Merwe, Elizabeth
Gwanyanya, Asfree
Kelly-Laubscher, Roisin
Journal Title
Journal ISSN
Volume Title
Publisher
John Wiley & Sons, Inc.
Published Version
Abstract
Objective: Sphingosine‐1‐phosphate is a natural metabolite that is cardioprotective, but its effects on endothelial glycocalyx damage during ischemia‐reperfusion are unknown. Therefore, we investigated the effect of sphingosine‐1‐phosphate on the endothelial glycocalyx during ischemia‐reperfusion. Methods: Isolated hearts from Wistar rats were perfused on a Langendorff system with Krebs‐Henseleit buffer and pretreated with sphingosine‐1‐phosphate (10 nmol/L) before ischemia‐reperfusion. Infarct size was measured by triphenyl tetrazolium chloride staining (n ≥ 6 per group). Cardiac edema was assessed by calculating total water content (n = 7 per group) and histologically quantifying the interstitial compartment (n ≥ 3 per group). The post‐ischemic coronary release of syndecan‐1 was quantified using ELISA. Syndecan‐1 immunostaining intensity was assessed in perfusion‐fixed hearts (n ≥ 3 per group). Results: Pretreatment with sphingosine‐1‐phosphate decreased infarct size in isolated hearts subjected to ischemia‐reperfusion (P = .01 vs ischemia‐reperfusion). However, sphingosine‐1‐phosphate had no effect on syndecan‐1 levels in the coronary effluent or on the intensity of the syndecan‐1 immunostaining signal in cardiac tissue. Heart total water content was not significantly different between control and ischemic groups but was significantly decreased in hearts treated with sphingosine‐1‐phosphate alone. Conclusion: These results suggest that sphingosine‐1‐phosphate‐induced cardioprotection against ischemia‐reperfusion injury is not mediated by the maintenance of syndecan‐1 in the endothelial glycocalyx.
Description
Keywords
Cardiac edema , Glycocalyx , Ischemia‐reperfusion , Myocardial infarction , S1P
Citation
Araibi, H., van der Merwe, E., Gwanyanya, A. and Kelly‐Laubscher, R. (2020) 'The effect of sphingosine‐1‐phosphate on the endothelial glycocalyx during ischemia‐reperfusion injury in the isolated rat heart', Microcirculation, 27(5), e12612 (11pp). doi: 10.1111/micc.12612
Link to publisher’s version
Copyright
© 2020, John Wiley & Sons, Ltd. This is the peer reviewed version of the following article: Araibi, H., van der Merwe, E., Gwanyanya, A. and Kelly‐Laubscher, R. (2020) 'The effect of sphingosine‐1‐phosphate on the endothelial glycocalyx during ischemia‐reperfusion injury in the isolated rat heart', Microcirculation, 27(5), e12612 (11pp), doi: 10.1111/micc.12612, which has been published in final form at https://doi.org/10.1111/micc.12612. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.