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RNA-Seq analysis of the guppy immune response against Gyrodactylus bullatarudis infection
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Date
2020-08-01
Authors
Konczal, Mateusz
Ellison, Amy R.
Phillips, Karl P.
Radwan, Jacek
Mohammed, Ryan S.
Cable, Joanne
Chadzinska, Magdalena
Journal Title
Journal ISSN
Volume Title
Publisher
John Wiley & Sons, Ltd.
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Abstract
Gyrodactylids are ubiquitous ectoparasites of teleost fish, but our understanding of the host immune response against them is fragmentary. Here, we used RNA-Seq to investigate genes involved in the primary response to infection with Gyrodactylus bullatarudis on the skin of guppies, Poecilia reticulata, an important evolutionary model, but also one of the most common fish in the global ornamental trade. Analysis of differentially expressed genes identified several immune-related categories, including IL-17 signalling pathway and Th17 cell differentiation, cytokine-cytokine receptor interaction, chemokine signalling pathway, NOD-like receptor signalling pathway, natural killer cell-mediated cytotoxicity and pathways involved in antigen recognition, processing and presentation. Components of both the innate and the adaptive immune responses play a role in response to gyrodactylid infection. Genes involved in IL-17/Th17 response were particularly enriched among differentially expressed genes, suggesting a significant role for this pathway in fish responses to ectoparasites. Our results revealed a sizable list of genes potentially involved in the teleost-gyrodactylid immune response.
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Keywords
Ectoparasites , Fish , Guppy , Gyrodactylus , Immunity , RNA-Seq , Th17 response , Transcriptome
Citation
Konczal, M., Ellison, A. R., Phillips, K. P., Radwan, J., Mohammed, R. S., Cable, J. and Chadzinska, M. (2020) 'RNA-Seq analysis of the guppy immune response against Gyrodactylus bullatarudis infection', Parasite Immunology. doi: 10.1111/pim.12782
Copyright
© 2020, John Wiley & Sons, Ltd. This is the peer reviewed version of the following article: Konczal, M., Ellison, A. R., Phillips, K. P., Radwan, J., Mohammed, R. S., Cable, J. and Chadzinska, M. (2020) 'RNA-Seq analysis of the guppy immune response against Gyrodactylus bullatarudis infection', Parasite Immunology, doi: 10.1111/pim.12782, which has been published in final form at https://doi.org/10.1111/pim.12782. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.