Electrochemical detection and capillary electrophoresis: Comparative studies for alkaline phosphatase (ALP) release from living cells

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dc.contributor.author Balbaied, Thanih
dc.contributor.author Hogan, Anna
dc.contributor.author Moore, Eric
dc.date.accessioned 2020-11-13T15:59:48Z
dc.date.available 2020-11-13T15:59:48Z
dc.date.issued 2020-08-11
dc.identifier.citation Balbaied, T., Hogan, A. and Moore, E. (2020) 'Electrochemical Detection and Capillary Electrophoresis: Comparative Studies for Alkaline Phosphatase (ALP) Release from Living Cells', Biosensors, 10(8), 95, (14 pp). doi: 10.3390/bios10080095 en
dc.identifier.volume 10 en
dc.identifier.issued 8 en
dc.identifier.startpage 1 en
dc.identifier.endpage 14 en
dc.identifier.issn 2079-6374
dc.identifier.uri http://hdl.handle.net/10468/10754
dc.identifier.doi 10.3390/bios10080095 en
dc.description.abstract Alkaline phosphatase (ALP) is one of the main biomarkers that is clinically detected in bone and liver disorders using optical assays. The electrochemical principle is important because point-of-care testing is increasing dramatically and absorbance techniques hardly compete with the medical revolution that is occurring. The detection of ALP using electrochemical detection is contributing to the integration systems field, and hence enhancing the detection of biological targets for pharmaceutical research and design systems. Moreover, in vitro electrochemical measurements use cost effective materials and simple techniques. Graphite screen-printed electrodes and linear sweep voltammetry were used to optimize the electrochemistry of the enzymatic product p-aminophenol using the enzyme kinetic assay. ALP release from embryonic and cancer cells was determined from adhesion cell culture. Additionally, capillary electrophoresis and colorimetric methods were applied for comparison assays. The resulting assays showed a dynamic range of ALP ranging from 1.5 to 1500 U/L, and limit of detection of 0.043 U/L. This was achieved by using 70 μL of the sample and an incubation time of 10 min at an optimal substrate concentration of 9.6 mM of p-aminophenol phosphate. A significant difference (p < 0.05) was measured between the absorbance assays. This paper demonstrates the advantages of the electrochemical assay for ALP release from cells, which is in line with recent trends in gene expression systems using microelectrode array technologies and devices for monitoring electrophysiological activity. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher MDPI en
dc.relation.uri https://www.mdpi.com/2079-6374/10/8/95
dc.rights © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.subject Electrochemistry en
dc.subject Linear sweep voltammetry en
dc.subject Alkaline phosphatase en
dc.subject Capillary electrophoresis en
dc.subject Colorimetry en
dc.subject Cancer cells en
dc.subject Embryonic cells en
dc.title Electrochemical detection and capillary electrophoresis: Comparative studies for alkaline phosphatase (ALP) release from living cells en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Thanih Balbaied, Tyndall National Institute, University College Cork, Cork, Ireland. +353-21-490-3000 en
dc.internal.availability Full text available en
dc.date.updated 2020-11-13T15:47:19Z
dc.description.version Published Version en
dc.internal.rssid 543756952
dc.contributor.funder Ministry of Education – Kingdom of Saudi Arabi en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Biosensors en
dc.internal.copyrightchecked Yes
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress eric.moore@tyndall.ie en
dc.internal.IRISemailaddress annamaria.hogan@ucc.ie en
dc.identifier.articleid 95 en


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© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited Except where otherwise noted, this item's license is described as © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
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