Resazurin-based assay for quantifying living cells during alkaline phosphatase (ALP) release

Show simple item record

dc.contributor.author Balbaied, Thanih
dc.contributor.author Moore, Eric
dc.date.accessioned 2020-11-13T16:08:56Z
dc.date.available 2020-11-13T16:08:56Z
dc.date.issued 2020-05-31
dc.identifier.citation Balbaied, T. and Moore, E. (2020) 'Resazurin-Based Assay for Quantifying Living Cells during Alkaline Phosphatase (ALP) Release', Applied Sciences, 10(11), 3840 (16 pp). doi: 10.3390/app10113840 en
dc.identifier.volume 10 en
dc.identifier.issued 11 en
dc.identifier.startpage 1 en
dc.identifier.endpage 16 en
dc.identifier.issn 2076-3417
dc.identifier.uri http://hdl.handle.net/10468/10755
dc.identifier.doi 10.3390/app10113840 en
dc.description.abstract Alkaline phosphatase (ALP) is an important reporter gene in the gene expression system, therefore monitoring cellular behavior including cell viability during ALP release is of significance. This assay produced a quantitative resazurin-based assay for cell viability in embryonic and cancer cells during alkaline phosphatase (ALP) release. A post-confluence culture method was applied to induce ALP in the cells of Balb/c 3T3, A549, MCF-7, and Ht-29. The density of each cell type was optimized using the standard cell culture assay. The main parameters affecting the results of resazurin involve the concentration of resazurin, incubation time, and cell number. The redox reaction, in which resazurin is reduced by the cells, was measured by fluorescence at 544 nm and 590 nm. The obtained data were compared with the hemocytometer assay. ALP release was determined using the optical active substrate p-nitrophenyl phosphate and colorimetric assay. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher MDPI en
dc.relation.uri https://www.mdpi.com/2076-3417/10/11/3840
dc.rights © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.subject Resazurin-based assays en
dc.subject Cell viability en
dc.subject Alkaline phosphatase en
dc.subject Balb/c 3T3 cells en
dc.subject A549 cells en
dc.subject MCF-7 cells en
dc.title Resazurin-based assay for quantifying living cells during alkaline phosphatase (ALP) release en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Thanih Balbaied, Chemistry, Tyndall National Institute, University College Cork, Cork, Ireland. +353-21-490-3000 en
dc.internal.availability Full text available en
dc.date.updated 2020-11-13T15:51:12Z
dc.description.version Published Version en
dc.internal.rssid 518801380
dc.contributor.funder Ministry of Education – Kingdom of Saudi Arabi en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Applied Sciences-Basel en
dc.internal.copyrightchecked Yes
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress eric.moore@tyndall.ie en
dc.identifier.articleid 3840 en


Files in this item

This item appears in the following Collection(s)

Show simple item record

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited Except where otherwise noted, this item's license is described as © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
This website uses cookies. By using this website, you consent to the use of cookies in accordance with the UCC Privacy and Cookies Statement. For more information about cookies and how you can disable them, visit our Privacy and Cookies statement