Effects of intracerebroventricular leptin and orexin-A on the baroreflex control of renal sympathetic nerve activity in conscious rats fed a normal or high-fat diet

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dc.contributor.author Huang, Chunlong
dc.contributor.author AlMarabeh, Sara
dc.contributor.author Cavers, Jeremy
dc.contributor.author Abdulla, Mohammed H.
dc.contributor.author Johns, Edward J.
dc.date.accessioned 2021-01-21T12:12:21Z
dc.date.available 2021-01-21T12:12:21Z
dc.date.issued 2020-12-22
dc.identifier.citation Huang, C., AlMarabeh, S., Cavers, J., Abdulla, M. H. and Johns, E. J. (2020) 'Effects of intracerebroventricular leptin and orexin-A on the baroreflex control of renal sympathetic nerve activity in conscious rats fed a normal or high-fat diet', Clinical and Experimental Pharmacology and Physiology. doi: 10.1111/1440-1681.13451 en
dc.identifier.issn 0305-1870
dc.identifier.uri http://hdl.handle.net/10468/10945
dc.identifier.doi 10.1111/1440-1681.13451 en
dc.description.abstract This study examined the effect of leptin and orexin‐A on autonomic baroreflex control in conscious Wistar rats exposed to high‐fat (45% fat) or normal (3.4%) diet for 4 weeks. Renal sympathetic nerve activity (RSNA), mean arterial pressure (MAP) and heart rate (HR) were monitored during the generation of baroreflex gain curves and acute volume expansion (VEP). Intracerebroventricular (ICV) leptin (1 μg/min) increased RSNA in the normal diet group (0.31 ± 0.04 vs 0.23 ± 0.03 mV/s) and MAP in the high‐fat diet group (115 ± 5 vs 105 ± 5 mm Hg, P < .05). Orexin‐A (50 ng/min) increased RSNA, HR and MAP in the high‐fat diet group (0.26 ± 0.03 vs 0.22 ± 0.02 mV/s, 454 ± 8 vs 417 ± 12 beats/min, 117 ± 1 vs 108 ± 1 mm Hg) and the normal diet group (0.18 ± 0.05 vs 0.17 ± 0.05 mV/s, 465 ± 10 vs 426 ± 6 beats/min, 116 ± 2 vs 104 ± 3 mm Hg). Baroreflex sensitivity for RSNA was increased during ICV leptin by 50% in the normal diet group, compared to 14% in the high‐fat diet group (P < .05). Similarly, orexin‐A increased baroreflex sensitivity by 56% and 50% in the high‐fat and normal diet groups, respectively (all P < .05). During ICV saline, VEP decreased RSNA by 31 ± 5% (P < .05) after 10 minutes and the magnitude of this response was blunted during ICV infusion of leptin (17 ± 2%, P < .05) but not orexin‐A in the normal diet group. RSNA response to VEP was not changed during ICV leptin or orexin‐A in the high‐fat diet group. These findings indicate possible central roles for leptin and orexin‐A in modulating the baroreflexes under normal or increased fat intake in conscious rats and potential therapeutic approaches for obesity associated hypertension. en
dc.description.sponsorship Health Research Board (Grant Number: RP/2007/17) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher John Wiley & Sons, Inc. en
dc.rights © 2020, John Wiley & Sons Australia, Ltd. This is the peer reviewed version of the following article: Huang, C., AlMarabeh, S., Cavers, J., Abdulla, M. H. and Johns, E. J. (2020) 'Effects of intracerebroventricular leptin and orexin-A on the baroreflex control of renal sympathetic nerve activity in conscious rats fed a normal or high-fat diet', Clinical and Experimental Pharmacology and Physiology, doi: 10.1111/1440-1681.13451, which has been published in final form at https://doi.org/10.1111/1440-1681.13451. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. en
dc.subject Baroreflex en
dc.subject Hypertension en
dc.subject Leptin en
dc.subject Volume expansion en
dc.subject High-fat diet en
dc.subject Orexin-A en
dc.title Effects of intracerebroventricular leptin and orexin-A on the baroreflex control of renal sympathetic nerve activity in conscious rats fed a normal or high-fat diet en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Mohammed Abdulla, Physiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: m.abdulla@ucc.ie en
dc.internal.availability Full text available en
dc.check.info Access to this article is restricted until 12 months after publication by request of the publisher. en
dc.check.date 2021-12-22
dc.date.updated 2021-01-21T11:59:27Z
dc.description.version Accepted Version en
dc.internal.rssid 550485497
dc.internal.wokid WOS:000600791200001
dc.contributor.funder Health Research Board en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Clinical and Experimental Pharmacology and Physiology en
dc.internal.copyrightchecked Yes
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress m.abdulla@ucc.ie en
dc.internal.bibliocheck In press. Check vol / issue / page range. Amend citation and copyright statement as necessary. en
dc.identifier.eissn 1440-1681


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