Bifidobacterium transformation

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dc.contributor.author Hoedt, Emily C.
dc.contributor.author Bongers, Roger S.
dc.contributor.author Bottacini, Francesca
dc.contributor.author Knol, Jan
dc.contributor.author MacSharry, John
dc.contributor.author van Sinderen, Douwe
dc.contributor.editor van Sinderen, Douwe
dc.contributor.editor Ventura, Marco
dc.date.accessioned 2021-03-19T12:27:39Z
dc.date.available 2021-03-19T12:27:39Z
dc.date.issued 2021-03-03
dc.identifier.citation Hoedt, Emily C.; Bongers, Roger S.; Bottacini, Francesca; Knol, Jan; MacSharry, John; van Sinderen, Douwe (2021) 'Bifidobacterium transformation', in van Sinderen, D. and Ventura, M. (eds) Bifidobacteria - Methods and Protocols. Methods in Molecular Biology, vol 2278, pp. 13-19. New York, NY: Humana Press. doi: 10.1007/978-1-0716-1274-3_2 en
dc.identifier.volume 2278 en
dc.identifier.startpage 13 en
dc.identifier.endpage 19 en
dc.identifier.isbn 978-1-0716-1273-6
dc.identifier.isbn 978-1-0716-1274-3
dc.identifier.issn 1064-3745
dc.identifier.uri http://hdl.handle.net/10468/11151
dc.identifier.doi 10.1007/978-1-0716-1274-3_2 en
dc.description.abstract The protocol presented in this chapter describes a generic method for electrotransformation of Bifidobacterium spp., outlining a technique that is ideal for conferring selective properties onto strains as well as allowing the user to introduce or knock out/in selected genes for phenotypic characterization purposes. We have generalized on the plasmid chosen for transformation and antibiotic selection marker, but the protocol is versatile in this respect and we are able to achieve transformation efficiencies up to 107 transformants/μg of DNA. en
dc.description.sponsorship Science Foundation Ireland (Grant Numbers SFI/12/RC/2273-P1; SFI/12/RC/2273-P2) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Springer Science+Business Media, LLC en
dc.relation.ispartof Bifidobacteria - Methods and Protocols
dc.relation.ispartof Methods in Molecular Biology, vol 2278
dc.rights © 2021, Springer Science+Business Media, LLC, part of Springer Nature. This is a post-peer-review, pre-copyedit version of a chapter published in van Sinderen, D. and Ventura, M. (eds) Bifidobacteria - Methods and Protocols. Methods in Molecular Biology, vol 2278, pp. 13-19. New York, NY: Humana Press. The final authenticated version is available online at: https://doi.org/10.1007/978-1-0716-1274-3_2 en
dc.subject Bifidobacterial en
dc.subject Electroporation en
dc.subject Genetic accessibility en
dc.subject Probiotic en
dc.title Bifidobacterium transformation en
dc.type Book chapter en
dc.internal.authorcontactother John Macsharry, Medical School Office, University College Cork, Cork, Ireland. +353-21-490-3000 Email: j.macsharry@ucc.ie en
dc.internal.availability Full text available en
dc.check.info Access to this chapter is restricted until 12 months after publication by request of the publisher. en
dc.check.date 2022-03-03
dc.date.updated 2021-03-19T12:11:37Z
dc.description.version Accepted Version en
dc.internal.rssid 559076542
dc.contributor.funder Nutricia Research Foundation en
dc.contributor.funder Science Foundation Ireland en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Methods in Molecular Biology en
dc.internal.copyrightchecked Yes
dc.internal.licenseacceptance Yes en
dc.internal.placepublication Cham en
dc.internal.IRISemailaddress j.macsharry@ucc.ie en
dc.internal.IRISemailaddress d.vansinderen@ucc.ie en
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/ en


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