The development of a ribosome profiling protocol to study translation in the yeast Yarrowia lipolytica

Abstract

Ribosome Profiling is an emerging technique that provides a genome wide-view of translation in-vivo, allowing an in-depth analysis of translation within a cell. It offers significant advantages over other techniques such as RNA-sequencing for interpreting transcriptional data about cells. Ribosome profiling can accelerate our understanding of complex biological processes happening within the cell and in turn, can be utilized to explore new ways for industrial exploitation of various cells. Here ribosome profiling, with the aid of a specialized ribo-seq data browser have been used to uncover new information about translation of the Yarrowia lipolytica transcriptome, an unusual yeast with interesting properties, with the aims of promoting its use as a candidate for biotechnology.