Exposure of Bifidobacterium longum subsp. infantis to milk oligosaccharides increases adhesion to epithelial cells and induces a substantial transcriptional response

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dc.contributor.author Kavanaugh, Devon W.
dc.contributor.author O'Callaghan, John
dc.contributor.author Butto, Ludovica F.
dc.contributor.author Slattery, Helen
dc.contributor.author Lane, Jonathan
dc.contributor.author Clyne, Marguerite
dc.contributor.author Kane, Marian
dc.contributor.author Joshi, Lokesh
dc.contributor.author Hickey, Rita M.
dc.contributor.editor Skurnik, Mikael
dc.date.accessioned 2013-07-04T08:12:03Z
dc.date.available 2013-07-04T08:12:03Z
dc.date.copyright 2013
dc.date.issued 2013-06-21
dc.identifier.citation Kavanaugh DW, O’Callaghan J, Butto´ LF, Slattery H, Lane J, et al. (2013) Exposure of Bifidobacterium longum subsp. infantis to Milk Oligosaccharides Increases Adhesion to Epithelial Cells and Induces a Substantial Transcriptional Response. PLoS ONE 8(6): e67224. doi:10.1371/journal.pone.0067224 en
dc.identifier.volume 8 en
dc.identifier.issued 6 en
dc.identifier.startpage e67224 en
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10468/1157
dc.identifier.doi 10.1371/journal.pone.0067224
dc.description.abstract In this study, we tested the hypothesis that milk oligosaccharides may contribute not only to selective growth of bifidobacteria, but also to their specific adhesive ability. Human milk oligosaccharides (39sialyllactose and 69sialyllactose) and a commercial prebiotic (Beneo Orafti P95; oligofructose) were assayed for their ability to promote adhesion of Bifidobacterium longum subsp. infantis ATCC 15697 to HT-29 and Caco-2 human intestinal cells. Treatment with the commercial prebiotic or 39sialyllactose did not enhance adhesion. However, treatment with 69sialyllactose resulted in increased adhesion (4.7 fold), while treatment with a mixture of 39- and 69-sialyllactose substantially increased adhesion (9.8 fold) to HT-29 intestinal cells. Microarray analyses were subsequently employed to investigate the transcriptional response of B. longum subsp. infantis to the different oligosaccharide treatments. This data correlated strongly with the observed changes in adhesion to HT-29 cells. The combination of 39- and 69-sialyllactose resulted in the greatest response at the genetic level (both in diversity and magnitude) followed by 69sialyllactose, and 39sialyllactose alone. The microarray data was further validated by means of real-time PCR. The current findings suggest that the increased adherence phenotype of Bifidobacterium longum subsp. infantis resulting from exposure to milk oligosaccharides is multi-faceted, involving transcription factors, chaperone proteins, adhesion-related proteins, and a glycoside hydrolase. This study gives additional insight into the role of milk oligosaccharides within the human intestine and the molecular mechanisms underpinning hostmicrobe interactions. en
dc.description.sponsorship Science Foundation Ireland (SFI-CSET); Science Foundation Ireland (08/SRC/B1393); Science Foundation Ireland (Alimentary Glycoscience Research Cluster (AGRC))Teagasc (Walsh Fellowship) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher PLOS en
dc.relation.uri http://dx.doi.org/10.1371%2Fjournal.pone.0067224
dc.rights © 2013 Kavanaugh et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. en
dc.rights.uri http://creativecommons.org/licenses/by/3.0/us/ en
dc.subject Human milk oligosaccharides en
dc.subject Bacterial adhesion en
dc.subject Bifidobacterium longum en
dc.title Exposure of Bifidobacterium longum subsp. infantis to milk oligosaccharides increases adhesion to epithelial cells and induces a substantial transcriptional response en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Ludovica F. Butto, Department of Microbiology, University College Cork, Cork, Ireland, Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder Teagasc en
dc.description.status Peer reviewed en
dc.identifier.journaltitle PLOS One en
dc.internal.copyrightchecked CORA - CC License en
dc.internal.IRISemailaddress j.ocallaghan@ucc.ie en


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©  2013 Kavanaugh et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Except where otherwise noted, this item's license is described as © 2013 Kavanaugh et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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