Genomic analysis of Leuconostoc citreum TR116 with metabolic reconstruction and the effects of fructose on gene expression for mannitol production

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dc.contributor.author Sahin, Aylin W.
dc.contributor.author Rice, Tom
dc.contributor.author Coffey, Aidan
dc.date.accessioned 2021-07-20T12:34:51Z
dc.date.available 2021-07-20T12:34:51Z
dc.date.issued 2021-07-03
dc.identifier.citation Sahin, A. W., Rice, T. and Coffey, A. (2021) 'Genomic analysis of Leuconostoc citreum TR116 with metabolic reconstruction and the effects of fructose on gene expression for mannitol production', International Journal of Food Microbiology, 354, 109327 (9pp). doi: 10.1016/j.ijfoodmicro.2021.109327 en
dc.identifier.volume 354 en
dc.identifier.startpage 1 en
dc.identifier.endpage 9 en
dc.identifier.issn 1879-3460
dc.identifier.issn 0168-1605
dc.identifier.uri http://hdl.handle.net/10468/11580
dc.identifier.doi 10.1016/j.ijfoodmicro.2021.109327 en
dc.description.abstract The species Leuconostoc citreum is often isolated from grain and vegetable fermentations such as sourdough, sauerkraut and kimchi. Lc. citreum has seen an increase in its use as a starter culture for various fermentations and food applications. The strain Lc. citreum TR116 has been applied previously in this laboratory aimed at sugar depletion through metabolism resulting in the reduction of fructose to mannitol, a polyol considered as a sweet carbohydrate. Besides reducing sugar, TR116 showed flavour modulating characteristics and contributes to the extension of microbial shelf life. In order to obtain a better understanding of this strain and to fully use its set of abilities, the genome of Lc. citreum TR116 was sequenced using the Illumina MiSeq, assembly with SPAdes and annotated by the Prokaryotic Genome Annotation Pipeline. Metabolic reconstruction was employed to elucidate carbohydrate, organic acid and amino acid metabolism in the strain. Of particular interest was the gene expression analysis ascertained the influence of fructose on the genes mdh and manX involved in the uptake of fructose and its conversion to mannitol. This investigation, the first in Lc. citreum, illustrates the metabolic processes involved in fermentation used by this strain and demonstrates that in the presence of fructose, expression of the genes mdh and manX is increased. The resulting transparency of the skill set of TR116 contributes highly to future functionalisation of food systems and food ingredients. en
dc.description.sponsorship Department of Agriculture, Food and the Marine, Ireland (Food Institutional Research Measure, project 14/F/803) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Elsevier B.V. en
dc.rights © 2021, Elsevier B.V. All rights reserved. This manuscript version is made available under the CC BY-NC-ND 4.0 license. en
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/ en
dc.subject Lactic acid bacteria en
dc.subject Genome sequence en
dc.subject Metabolism en
dc.subject Carbohydrate en
dc.subject Mannitol dehydrogenase en
dc.subject Sugar reduction en
dc.title Genomic analysis of Leuconostoc citreum TR116 with metabolic reconstruction and the effects of fructose on gene expression for mannitol production en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Aylin Sahin, Food & Nutritional Sciences, University College Cork, Cork, Ireland. +353-21-490-3000 Email: aylin.sahin@ucc.ie en
dc.internal.availability Full text available en
dc.check.info Access to this article is restricted until 12 months after publication by request of the publisher. en
dc.check.date 2022-07-03
dc.date.updated 2021-07-20T12:24:39Z
dc.description.version Accepted Version en
dc.internal.rssid 574895806
dc.internal.pmid 34247022
dc.contributor.funder Department of Agriculture, Food and the Marine, Ireland en
dc.description.status Peer reviewed en
dc.identifier.journaltitle International Journal of Food Microbiology en
dc.internal.copyrightchecked Yes
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress aylin.sahin@ucc.ie en
dc.identifier.articleid 109327 en


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© 2021, Elsevier B.V. All rights reserved. This manuscript version is made available under the CC BY-NC-ND 4.0 license. Except where otherwise noted, this item's license is described as © 2021, Elsevier B.V. All rights reserved. This manuscript version is made available under the CC BY-NC-ND 4.0 license.
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