Omics based approaches for the identification of novel bioactive secondary metabolites from marine sponge derived bacterial isolates

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dc.contributor.advisor Dobson, Alan en Patry, Sloane 2021-09-10T11:09:03Z 2021-09-10T11:09:03Z 2021-02 2021-02
dc.identifier.citation Patry, S. A. D. 2021. Omics based approaches for the identification of novel bioactive secondary metabolites from marine sponge derived bacterial isolates. PhD Thesis, University College Cork. en
dc.identifier.endpage 325 en
dc.description.abstract Antibiotic resistance is a major threat to public health worldwide which, urgently requires the discovery and development of novel antimicrobial drugs with new modes of action. The principal goal of this project is the discovery of new bioactive secondary metabolites that may lead to the discovery of new antibiotics to help fight against the antibiotic resistance crisis. The marine environment is a unique habitat where deep-sea microorganisms, because of their ability to adapt to this extreme environment, have the potential to produce novel secondary metabolites with potent biological activities. Furthermore, microbes associated with marine sponges are exposed to highly competitive environments both physiologically and nutritionally which is likely to promote the production of novel secondary metabolites. The genus Streptomyces produces secondary metabolic compounds that are rich in biological activity and marine Streptomyces strains host abundant small molecule biosynthetic gene clusters (smBGCs) which encode polyketides, Non ribosomal peptide synthases (NRPS), siderophore, bacteriocin and lantipeptides. In this respect, by focusing on Streptomyces samples taken from sponges in marine environments off the Irish coast, we aimed to characterize new marine derived bacterial strains and to explore their bioactive potential. In order to maximize the number of potential novel bioactive metabolites that could be be identified from each Streptomyces strain, and to generate the most detailed information underpinning their associated metabolic pathways, the One Strain MAny Compounds (OSMAC) approach, combined with a multi-omics approach, was employed in this project. In total, the Streptomyces isolates (B226SN104, SM3 and SM9), produced 612 metabolites including but not limited to, Bisucaberin B, Maculosin, Desferrioxamine B, Desferrioxamine E, Concanamycin B, Lipoamicoumacin B, Probestin, Surugamide A, Fiscalin B, Gibbestatin B and Antimycin A1. The Streptomyces isolates produced metabolites active against the growth 21 of antimicrobial-resistant microorganisms categorized as an “Urgent” or “Serious” threat by the World Health Organization (WHO), including Acinetobacter baumannii, Pseudomonas aeruginosa, Staphylococcus aureus and Candida spp. In addition, other activities of biomedical interest were identified, namely anti-cancer, anti-inflammatory and antioxidant activities. Furthermore, the omics approach allowed us to predict 19 potential bioactive and potential novel promising molecules from the Streptomyces SM9 isolate. Taken together, this project demonstrated the significant potential the modified OSMAC/multi omics approach could have in expanding the number of novel secondary and bioactive metabolites that could be generated from existing environmental microbial isolates. Furthermore, it has contributed to the fight against antimicrobial resistance by identifying as of yet potential undiscovered molecules with antimicrobial potential. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher University College Cork en
dc.rights © 2021, Sloane Anaϊs Deborah Patry. en
dc.rights.uri en
dc.subject OSMAC en
dc.subject Omics en
dc.subject Bioinformatics en
dc.subject LC-MS/MS en
dc.subject Marine Streptomyces en
dc.subject Secondary metabolites en
dc.subject Natural product discovery en
dc.subject Molecular network en
dc.subject Bioactive metabolites en
dc.subject Antimicrobial resistance crisis en
dc.subject Metabolomics en
dc.subject Microbiology en
dc.subject Genomics en
dc.subject Bioactive molecular network en
dc.subject Crude extracts en
dc.subject Biosynthetic gene clusters sm(BGC) en
dc.subject Bioactivity screening en
dc.title Omics based approaches for the identification of novel bioactive secondary metabolites from marine sponge derived bacterial isolates en
dc.type Doctoral thesis en
dc.type.qualificationlevel Doctoral en
dc.type.qualificationname PhD - Doctor of Philosophy en
dc.internal.availability Full text available en
dc.description.version Accepted Version en
dc.contributor.funder Horizon 2020 en
dc.description.status Not peer reviewed en Microbiology en
dc.internal.conferring Autumn 2021 en
dc.internal.ricu Environmental Research Institute (ERI) en
dc.internal.ricu Food Institute en
dc.relation.project info:eu-repo/grantAgreement/EC/H2020::MSCA-ITN-ETN/721421/EU/Improving the flow in the pipeline of the next generation of marine biodiscovery scientists/MarPipe en
dc.availability.bitstream openaccess

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© 2021, Sloane Anaϊs Deborah Patry. Except where otherwise noted, this item's license is described as © 2021, Sloane Anaϊs Deborah Patry.
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