Antimicrobial activities and diversity of sponge derived microbes

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dc.contributor.advisor Dobson, Alan D. W. en
dc.contributor.advisor O'Gara, Fergal en Flemer, Burkhardt 2013-07-17T12:32:35Z 2013-07-17T12:32:35Z 2013 2013
dc.identifier.citation Flemer, B. 2013. Antimicrobial activities and diversity of sponge derived microbes. PhD Thesis, University College Cork. en
dc.identifier.endpage 204
dc.description.abstract In this study, marine sponges collected in Irish waters were analysed for their associated microbiota. Of the approximately 240 bacterial isolates obtained from two sponges several showed antimicrobial activity; among them members of genera which have rarely been shown to produce antimicrobial compounds. Differences observed from the sponge-derived groups of isolates in terms of bioactivity suggests that S. carnosus isolates may be a better source of antibacterial compounds, while Leucosolenia sp. isolates appear to be a better source of antifungal compounds. More than 60% of fungal isolates obtained from 12 sponge samples proved to be bioactive. One of the isolates, which was closely related to Fusarium oxysporum and showed activity against bacteria and fungi, was investigated for its secondary metabolite genes. At least 5 different NRPS genes, with a sequence similarity as low as 50 % to known genes, were identified highlighting the likelihood that this isolate may be capable of producing novel secondary metabolites. A Micromonospora sp. was isolated from a Haliclona simulans sample collected in Irish waters. The isolate inhibited the growth of Gram positive bacterial test strains in three different antimicrobial assays. Employing preparative layer chromatography the compound responsible for the bioactivity could be isolated. According to LC-MS andNMR data the bioactive compound could indeed be novel. Finally, two deep water sponges were shown to host a remarkably different bacterial and archaeal diversity by application of 454 Pyrosequencing. The L. diversichela –proteobacterial community was dominated by a single ƴ-proteobacterial bacterium whereas the S. normani sample hosted a largely sponge specific microbial community, even more diverse than has been previously reported for shallow water sponges. Organisms potentially involved in nitrification, sulphate reduction and secondary metabolite production were found to be spatially distributed in the sponge. Furthermore, a deep sea specific population was implied. en
dc.description.sponsorship Marine Institute (Beaufort Marine Biodiscovery Award (BEAU/BIOD/01)) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher University College Cork en
dc.rights © 2013, Burkhardt Flemer en
dc.rights.uri en
dc.subject Molecular microbial diversity en
dc.subject 454 sequencing en
dc.subject Marine sponges en
dc.subject Bioactive secondary metabolites en
dc.subject.lcsh Sponges en
dc.title Antimicrobial activities and diversity of sponge derived microbes en
dc.type Doctoral thesis en
dc.type.qualificationlevel Doctoral en
dc.type.qualificationname PhD (Science) en
dc.internal.availability Full text available en No embargo required en
dc.description.version Accepted Version
dc.contributor.funder Marine Institute en
dc.description.status Not peer reviewed en Environmental Research Institute en
dc.check.type No Embargo Required
dc.check.reason No embargo required en
dc.check.opt-out Not applicable en
dc.thesis.opt-out false *
dc.check.embargoformat Not applicable en
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