Enamel remineralization via poly(amido amine) and adhesive resin containing calcium phosphate nanoparticles

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Gao, Yuan
Liang, Kunneng
Weir, Michael D.
Gao, Jianghong
Imazato, Satoshi
Tay, Franklin R.
Lynch, Christopher D.
Oates, Thomas W.
Li, Jiyao
Xu, Hockin H.K.
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Objectives:The objective of this study was to investigate enamel remineralization using salivary statherin pro-tein-inspired poly(amidoamine) dendrimer (SN15-PAMAM) and adhesive containing nanoparticles of amor-phous calcium phosphate (NACP) in a cyclic artificial saliva/demineralizing solution for thefirst time.Methods:The enamel shear bond strengths of NACP adhesives were measured compared to commercial adhesive(Scotchbond Multi-Purpose, 3 M). Adhesive disks containing NACP were tested for calcium (Ca) and phosphorus(P) ions release. Four groups were tested: (1) enamel control, (2) enamel with NACP, (3) enamel with SN15-PAMAM, and (4) enamel with SN15-PAMAM + NACP. The specimens were treated with cyclic artificial saliva/demineralizing solution for 28 days. The remineralized enamel specimens were examined by surface and cross-sectional hardness test.Results:NACP adhesive yielded a similar shear bond strength to commercial control (Scotchbond Multi-Purpose,3 M). NACP adhesive released high levels of Ca and P ions. At 28 days, the enamel hardness of SN15-PAMAM +NACP group was 2.89 ± 0.13 GPa, significantly higher than that of control group (1.46 ± 0.10 GPa) (p< 0.05).SN15-PAMAM + NACP increased the enamel cross-sectional hardness at 28 days; at 25μm, enamel cross-sectional hardness was 90 % higher than that of control group (p< 0.05).Significance:The novel SN15-PAMAM + NACP adhesive method could achieve 90 % higher enamel reminer-alization of the artificial caries than the control under acid challenge for thefirst time. This method is promisingfor use after tooth cavity preparation, or as a coating on enamel with white spot lesions (WSLs) for prevention, toreduce secondary caries, prevent caries procession and protect tooth structures.
Enamel remineralization , Statherin protein poly(amidoamine) dendrimer , Adhesive , pH cycling , Nano-calcium phosphate , Enamel hardness
Semple, C. J., Rutherford, H., Killough, S., Moore, C. and McKenna, G. (2019) 'Enamel remineralization via poly(amido amine) and adhesive resin containing calcium phosphate nanoparticles', Journal of Dentistry, 92, 103262 (8pp). doi:10.1016/j.jdent.2019.103262