Lipidome analysis in brain and peripheral plasma following milk fat globule membrane supplementation in rodents
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Date
2022-09-06
Authors
Davies, R.
van Diepen, J. A.
Brink, L. R.
Bijlsma, S.
Neufeld, K. M.
Cryan, John F.
O'Mahony, Siobhain M.
Bobeldijk, I.
Gross, G.
Journal Title
Journal ISSN
Volume Title
Publisher
Wiley
Published Version
Abstract
Scope: Milk fat globule membrane (MFGM) is an essential component of milk. Bovine MFGM (bMFGM) has been shown to support cognitive development and increase relative concentrations of serum phospholipids. This study investigated bioavailability of bMFGM components after oral administration in two preclinical models to explore whether dietary bMFGM induced parallel changes to plasma and brain lipidomes. Methods and results: Transgenic APOE*3.Leiden mice (n = 18/group) and Sprague-Dawley rats (n = 12/group) were fed bMFGM-enriched (MFGM+) or Control diet, after which phospholipid profiles were determined in peripheral plasma, hippocampus and prefrontal cortex tissue by targeted mass spectrometry. Multivariate analysis of lipidomic profiles demonstrated a clear separation between MFGM+ and Control plasma samples across rodents. In plasma, sphingomyelins contributed the most to the separation of lipid patterns among both models, where three sphingomyelins (d18:1/14:0, d18:1/23:0, d18:1/23:1[9Z]) were significantly and consistently higher in the circulation of MFGM+ versus Control groups. A similar trend was observed in rat prefrontal cortex, although no significant separation of the brain lipidome was demonstrated. Conclusion: bMFGM-enriched diet alters plasma phospholipid composition in rodents, predominantly increasing sphingomyelin levels in the systemic circulation with some similar, but non-significant, trends in central regions of the brain. These changes may contribute to the beneficial effects of dietary bMFGM on neurodevelopment during early life.
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Keywords
Milk fat globule membrane , Lipidomics , Blood plasma , Brain , Rodents
Citation
Davies, R., van Diepen, J.A., Brink, L.R., Bijlsma, S., Neufeld, K.M., Cryan, J.F., O’Mahony, S.M., Bobeldijk, I. and Gross, G. (2022) ‘Lipidome analysis in brain and peripheral plasma following milk fat globule membrane supplementation in rodents’, Molecular Nutrition & Food Research, 2200177, doi: 10.1002/mnfr.202200177
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© 2022 WILEY-VCH Verlag GmbH & Co. KGaA. This is the peer reviewed version of the following article: Lipidome analysis in brain and peripheral plasma following milk fat globule membrane supplementation in rodents. Mol. Nutr. Food Res. 2022, 2200177, which has been published in final form at https://doi.org/10.1002/mnfr.202200177. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.