Proteomic approach to oxidative stress in Daphnia magna

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dc.contributor.advisor Sheehan, David en Rainville, Louis-Charles 2014-04-02T15:59:46Z 2014-04-02T15:59:46Z 2014 2014
dc.identifier.citation Rainville, L.-C. 2014. Proteomic approach to oxidative stress in Daphnia magna. PhD Thesis, University College Cork. en
dc.identifier.endpage 125
dc.description.abstract The keystone aquatic organism Daphnia magna is extensively used to assess the toxicity of chemicals. This has recently lead to an increase in the omics literature focusing on daphnids, an increase fuelled by the sequencing of the Daphnia pulex genome. Yet, no omics study has looked directly at oxidative stress (OS) in daphnids, even though OS is of primary importance in the response of aquatic organisms to their changing environment and is often induced by anthropogenic xenobiotics. This thesis thus focuses on the application of redox-proteomics, the study of the oxidative modification of proteins, to D. magna Specifically, daphnids were exposed to copper or paraquat, two well studied prooxidants, and protein carbonyls were labelled with fluorescein-5-thiosemicarbazide prior to twodimensional electrophoresis (2DE). This showed clearly that both compounds affect a different portion of the proteome. The identified proteins indicated that energy metabolism was affected by paraquat, while copper induced a reduction of the heat shock response (heat shock proteins, proteases and chaperones) a counterintuitive result which may be adaptative to metal toxicity in arthropods. The same approach was then applied to the study of the toxicity mechanism of silver nanoparticles (AgNP), an increasingly utilised form of silver with expected environmental toxicity, and its comparison to silver nitrate. The results demonstrate that, although less toxic than silver ions, AgNP toxicity functions through a different mechanism. AgNP toxicity is thus not a product of silver dissolution and increased protein carbonylation indicates that AgNP cause OS. Interestingly three of the four tested compounds altered vitellogenin levels and oxidation. Vitellogenins could thus represent an interesting subproteome for the detection of stress in daphnids. Finally, an experiment with oxidised BSA demonstrates the applicability of solid phase hydrazide in the enrichment of undigested carbonylated proteins. en
dc.description.sponsorship Irish Research Council for Science Engineering and Technology (EMBARK initiative) en
dc.description.sponsorship Fonds Québécois de la Recherche sur la Nature et les Technologies (Bourse de Doctorat) fr
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher University College Cork en
dc.rights © 2014, Louis-Charles Rainville en
dc.rights.uri en
dc.subject Oxidative stress en
dc.subject Ecotoxicology en
dc.subject Nanoparticles en
dc.subject Solid-phase hydrazide en
dc.subject Heat shock response en
dc.subject.lcsh Pollution--Environmental aspects en
dc.subject.lcsh Daphnia magna en
dc.subject.lcsh Proteomics en
dc.title Proteomic approach to oxidative stress in Daphnia magna en
dc.type Doctoral thesis en
dc.type.qualificationlevel Doctoral en
dc.type.qualificationname PhD (Science) en
dc.internal.availability Full text available en No embargo required en
dc.description.version Accepted Version
dc.contributor.funder Irish Research Council for Science Engineering and Technology en
dc.contributor.funder Fonds Québécois de la Recherche sur la Nature et les Technologies, Canada fr
dc.description.status Not peer reviewed en Biochemistry en
dc.check.type No Embargo Required
dc.check.reason No embargo required en
dc.check.opt-out Not applicable en
dc.thesis.opt-out false
dc.check.embargoformat Not applicable en
dc.internal.conferring Summer Conferring 2014

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© 2014, Louis-Charles Rainville Except where otherwise noted, this item's license is described as © 2014, Louis-Charles Rainville
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