The impact of host-microbe interactions on murine colonic secretomotor function

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dc.contributor.advisor Hyland, Niall P. en
dc.contributor.advisor Cryan, John F. en
dc.contributor.author Lomasney, Kevin W.
dc.date.accessioned 2015-10-20T10:45:40Z
dc.date.available 2015-10-20T10:45:40Z
dc.date.issued 2014
dc.date.submitted 2014
dc.identifier.citation Lomasney, K. W. 2014. The impact of host-microbe interactions on murine colonic secretomotor function. PhD Thesis, University College Cork. en
dc.identifier.endpage 206
dc.identifier.uri http://hdl.handle.net/10468/2002
dc.description.abstract The overall objective of this thesis was to gain further insight into the mechanisms underlying commensal microbial influences on intestinal ion transport. In this regard, I examined the impact of commensal host-microbe interactions on colonic secretomotor function in mouse. I first examined the influence of two different probiotic (microorganisms which, when given in adequate amounts, can confer health benefits upon the host) strains, Bifidobacterium infantis 35624 and L. salivarius UCC118 on active colonic ion transport in the mouse, using the Ussing Chamber. I found that both probiotics appear to have converging effects on ion transport at a functional level. However, L. salivarius UCC118 may preferentially inhibit neurally-evoked ion transport. Next I examined the impact of the host microbiota itself on both baseline and stimulated colonic secretomotor function as well as probiotic induced changes in ion transport. I provide further evidence that the microbiota is capable of mediating alterations in colonic ion transport, and specifically suggests that it can influence cAMP-mediated responses. Finally, it has been well documented that many probiotics elicit their effects via secreted bioactives, therefore, I studied the effects of microbially produced GABA, contained in supernatants from the commensal microbe Lactobacillus brevis DPC6108, on colonic secretomotor function. In conclusion, I believe that commensal microbes have an important and strain specific functional influence on colonic ion transport and secretomotor function and these effects can be mediated via extracellular bioactives. Moreover, I believe that functional ex-vivo studies such as those carried out in this thesis have a critical role to play in our future understanding of host-microbe interactions in the gut. en
dc.description.sponsorship Science Foundation Ireland (Alimentary Pharmabiotic Centre Funding) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher University College Cork en
dc.rights © 2014, Kevin W. Lomasney. en
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/ en
dc.subject Microbiota en
dc.subject Probiotics en
dc.subject Intestine en
dc.subject Mouse en
dc.subject Ion transport en
dc.subject Enteric nervous system en
dc.subject Ussing chamber en
dc.title The impact of host-microbe interactions on murine colonic secretomotor function en
dc.type Doctoral thesis en
dc.type.qualificationlevel Doctoral en
dc.type.qualificationname PhD (Medicine and Health) en
dc.internal.availability Full text available en
dc.check.info No embargo required en
dc.description.version Accepted Version
dc.contributor.funder Science Foundation Ireland en
dc.description.status Not peer reviewed en
dc.internal.school Alimentary Pharmabotic Centre en
dc.internal.school Pharmacology and Therapeutics en
dc.check.type No Embargo Required
dc.check.reason No embargo required en
dc.check.opt-out Not applicable en
dc.thesis.opt-out false
dc.check.embargoformat Not applicable en
ucc.workflow.supervisor n.hyland@ucc.ie
dc.internal.conferring Spring Conferring 2015


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© 2014, Kevin W. Lomasney. Except where otherwise noted, this item's license is described as © 2014, Kevin W. Lomasney.
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