Cross-feeding by Bifidobacterium breve UCC2003 during co-cultivation with Bifidobacterium bifidum PRL2010 in a mucin-based medium

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dc.contributor.author Egan, Muireann
dc.contributor.author O'Connell Motherway, Mary
dc.contributor.author Kilcoyne, Michelle
dc.contributor.author Kane, Marian
dc.contributor.author Joshi, Lokesh
dc.contributor.author Ventura, Marco
dc.contributor.author van Sinderen, Douwe
dc.date.accessioned 2016-01-20T10:41:57Z
dc.date.available 2016-01-20T10:41:57Z
dc.date.issued 2014-11-25
dc.identifier.citation EGAN, M., O’CONNELL MOTHERWAY, M., KILCOYNE, M., KANE, M., JOSHI, L., VENTURA, M. & VAN SINDEREN, D. 2014. Cross-feeding by Bifidobacterium breve UCC2003 during co-cultivation with Bifidobacterium bifidum PRL2010 in a mucin-based medium. BMC Microbiology, 14:282, 1-14. http://dx.doi.org/10.1186/s12866-014-0282-7 en
dc.identifier.volume 14 en
dc.identifier.startpage 1 en
dc.identifier.endpage 14 en
dc.identifier.issn 1471-2180
dc.identifier.uri http://hdl.handle.net/10468/2198
dc.identifier.doi 10.1186/s12866-014-0282-7
dc.description.abstract BACKGROUND: Bifidobacteria constitute a specific group of commensal bacteria that commonly inhabit the mammalian gastrointestinal tract. Bifidobacterium breve UCC2003 was previously shown to utilize a variety of plant/diet/host-derived carbohydrates, including cellodextrin, starch and galactan, as well as the mucin and HMO-derived monosaccharide, sialic acid. In the current study, we investigated the ability of this strain to utilize parts of a host-derived source of carbohydrate, namely the mucin glycoprotein, when grown in co-culture with the mucin-degrading Bifidobacterium bifidum PRL2010. RESULTS: B. breve UCC2003 was shown to exhibit growth properties in a mucin-based medium, but only when grown in the presence of B. bifidum PRL2010, which is known to metabolize mucin. A combination of HPAEC-PAD and transcriptome analyses identified some of the possible monosaccharides and oligosaccharides which support this enhanced co-cultivation growth/viability phenotype. CONCLUSION: This study describes the potential existence of a gut commensal relationship between two bifidobacterial species. We demonstrate the in vitro ability of B. breve UCC2003 to cross-feed on sugars released by the mucin-degrading activity of B. bifidum PRL2010, thus advancing our knowledge on the metabolic adaptability which allows the former strain to colonize the (infant) gut by its extensive metabolic abilities to (co-)utilize available carbohydrate sources. en
dc.description.sponsorship Science Foundation Ireland ((SFI 07/CE/B1368), (SFI/12/RC/2273),( SFI 08/SRC/B1393)); European Commission (EU FP7 programme (Grant no. 260600)); Health Research Board (HRB postdoctoral fellowship (Grant No. PDTM/20011/9)) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Biomed Central Ltd. en
dc.rights © 2014 Egan et al.; licensee BioMed Central Ltd., 2014. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. en
dc.rights.uri http://creativecommons.org/licenses/by/4.0/ en
dc.subject Bifidobacteria en
dc.subject Probiotic en
dc.subject Fucose en
dc.subject Sialic acid en
dc.subject Galactose en
dc.subject Human milk en
dc.subject Oligosaccharides en
dc.subject Human colon ecosystems en
dc.subject DNA microarray data en
dc.subject Carbohydrate en
dc.subject Anaerobic bacteria en
dc.subject Intestinal tract en
dc.subject Gene expression en
dc.subject O-glycosylation en
dc.subject Genome analysis en
dc.subject Leloir pathway en
dc.title Cross-feeding by Bifidobacterium breve UCC2003 during co-cultivation with Bifidobacterium bifidum PRL2010 in a mucin-based medium en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Douwe van Sinderen, Microbiology, University College Cork, Cork, Ireland. +353-21-490-1365 Email: d.vansinderen@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Health Research Board en
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder European Commission en
dc.description.status Peer reviewed en
dc.identifier.journaltitle BMC Microbiology en
dc.internal.copyrightchecked Open Access articles licensed via CC-BY 4.0 with UCC affiliated authors. Uploaded Jan 2016. en
dc.identifier.articleid 282


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© 2014 Egan et al.; licensee BioMed Central Ltd., 2014. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. Except where otherwise noted, this item's license is described as © 2014 Egan et al.; licensee BioMed Central Ltd., 2014. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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