Cross-feeding by Bifidobacterium breve UCC2003 during co-cultivation with Bifidobacterium bifidum PRL2010 in a mucin-based medium

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Date
2014-11-25
Authors
Egan, Muireann
O'Connell Motherway, Mary
Kilcoyne, Michelle
Kane, Marian
Joshi, Lokesh
Ventura, Marco
van Sinderen, Douwe
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Biomed Central Ltd.
Published Version
10.1186/s12866-014-0282-7
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Abstract
BACKGROUND: Bifidobacteria constitute a specific group of commensal bacteria that commonly inhabit the mammalian gastrointestinal tract. Bifidobacterium breve UCC2003 was previously shown to utilize a variety of plant/diet/host-derived carbohydrates, including cellodextrin, starch and galactan, as well as the mucin and HMO-derived monosaccharide, sialic acid. In the current study, we investigated the ability of this strain to utilize parts of a host-derived source of carbohydrate, namely the mucin glycoprotein, when grown in co-culture with the mucin-degrading Bifidobacterium bifidum PRL2010. RESULTS: B. breve UCC2003 was shown to exhibit growth properties in a mucin-based medium, but only when grown in the presence of B. bifidum PRL2010, which is known to metabolize mucin. A combination of HPAEC-PAD and transcriptome analyses identified some of the possible monosaccharides and oligosaccharides which support this enhanced co-cultivation growth/viability phenotype. CONCLUSION: This study describes the potential existence of a gut commensal relationship between two bifidobacterial species. We demonstrate the in vitro ability of B. breve UCC2003 to cross-feed on sugars released by the mucin-degrading activity of B. bifidum PRL2010, thus advancing our knowledge on the metabolic adaptability which allows the former strain to colonize the (infant) gut by its extensive metabolic abilities to (co-)utilize available carbohydrate sources.
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Keywords
Bifidobacteria , Probiotic , Fucose , Sialic acid , Galactose , Human milk , Oligosaccharides , Human colon ecosystems , DNA microarray data , Carbohydrate , Anaerobic bacteria , Intestinal tract , Gene expression , O-glycosylation , Genome analysis , Leloir pathway
Citation
EGAN, M., O’CONNELL MOTHERWAY, M., KILCOYNE, M., KANE, M., JOSHI, L., VENTURA, M. & VAN SINDEREN, D. 2014. Cross-feeding by Bifidobacterium breve UCC2003 during co-cultivation with Bifidobacterium bifidum PRL2010 in a mucin-based medium. BMC Microbiology, 14:282, 1-14. http://dx.doi.org/10.1186/s12866-014-0282-7
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https://hdl.handle.net/10468/2198
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Microbiology - Journal Articles
APC Microbiome Ireland - Journal Articles
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© 2014 Egan et al.; licensee BioMed Central Ltd., 2014. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.