Positively charged, surfactant-free gold nanoparticles for nucleic acid delivery

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dc.contributor.author Guo, Jianfeng
dc.contributor.author Armstrong, Mark J.
dc.contributor.author O'Driscoll, Caitríona M.
dc.contributor.author Holmes, Justin D.
dc.contributor.author Rahme, Kamil
dc.date.accessioned 2016-01-26T12:10:16Z
dc.date.available 2016-01-26T12:10:16Z
dc.date.issued 2015-02-03
dc.identifier.citation GUO, J., ARMSTRONG, M. J., O'DRISCOLL, C. M., HOLMES, J. D. & RAHME, K. 2015. Positively charged, surfactant-free gold nanoparticles for nucleic acid delivery. RSC Advances, 5, 17862-17871.http://dx.doi.org/10.1039/C4RA16294C en
dc.identifier.volume 5 en
dc.identifier.issued 23 en
dc.identifier.startpage 17862 en
dc.identifier.endpage 17871 en
dc.identifier.issn 2046-2069
dc.identifier.uri http://hdl.handle.net/10468/2216
dc.identifier.doi 10.1039/C4RA16294C
dc.description.abstract Positively charged, surfactant-free gold nanoparticles (Au NPs) with diameters ranging between 2-200 nm have been synthesised in water via a seed-mediated growth method, involving the reduction of gold(iii) chloride (AuCl3) by hydroxylamine hydrochloride (NH2OH[middle dot]HCl) in the presence of l-cysteine methyl ester hydrochloride (HSCH2CH(NH2)COOCH3[middle dot]HCl) as a capping agent. The mercapto group (-SH) on the capping ligand has a high affinity for Au, anchoring the cysteine group to the nanoparticles, whilst the ammonium group (-NH3+), formed by the presence of an amine group in slightly acidic media (pH [similar] 4.5-5), resulted in positively charged colloidal nanoparticles ([small zeta]-potential +33 to +49 mV), which was key to their electrostatic stability. Data from cytotoxicity studies performed on a range of different cell types (human and murine), including human prostate cancer cells (PC3), showed that the positively charged Au-l-cysteine-cysteine nanoparticles were less cytotoxic than positively charged Au NPs produced using commonly employed surfactant cetyl trimethyl ammonium bromide (CTAB) under similar conditions. In addition, the positively charged Au NPs could be successfully complexed with small interfering RNA (siRNA). At the cellular level, the uptake of fluorescein siRNA from the charged nanoparticles was comparable to uptake from the commercial carrier INTERFRin[trade mark sign], implying the potential application of these novel vectors for nucleic acid delivery. en
dc.description.sponsorship Irish Research Council (Government of Ireland Postdoctoral Fellowship GOIPD/2013/150) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Royal Society of Chemistry en
dc.rights © 2015, the Authors. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. en
dc.rights.uri http://creativecommons.org/licenses/by/3.0/ en
dc.subject Amino acids en
dc.subject Chlorine compounds en
dc.subject Cytotoxicity en
dc.subject Gold alloys en
dc.subject Metal nanoparticles en
dc.subject Nanoparticles en
dc.subject Nucleic acids en
dc.subject Nucleic acid deliveries en
dc.subject Cetyl trimethyl ammonium bromide en
dc.subject CTAB en
dc.subject Human prostate cancer cells en
dc.subject Hydroxylamine hydrochloride en
dc.subject L-Cysteine methyl esters en
dc.subject Seed-mediated growth methods en
dc.subject Small interfering rna (siRNA) en
dc.subject Diseases en
dc.subject Dyes en
dc.subject Fiber optic sensors en
dc.subject Mobile security en
dc.subject Surface active agents en
dc.subject Electrostatic stability en
dc.title Positively charged, surfactant-free gold nanoparticles for nucleic acid delivery en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Justin D. Holmes, Department of Chemistry, College of Science, Engineering and Food Science, University College Cork, Cork, Ireland. +353-21-490-3000 Email: j.holmes@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Irish Research Council
dc.description.status Peer reviewed en
dc.identifier.journaltitle RSC Advances en
dc.internal.IRISemailaddress j.holmes@ucc.ie en


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© 2015, the Authors. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Except where otherwise noted, this item's license is described as © 2015, the Authors. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
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