Mangotoxin production of Pseudomonas syringae pv. syringae is regulated by MgoA

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dc.contributor.author Carrión, Víctor J.
dc.contributor.author van der Voort, Menno
dc.contributor.author Arrebola, Eva
dc.contributor.author Gutiérrez-Barranquero, José A.
dc.contributor.author de Vicente, Antonio
dc.contributor.author Raaijmakers, Jos M.
dc.contributor.author Cazorla, Francisco M.
dc.date.accessioned 2016-02-02T16:12:27Z
dc.date.available 2016-02-02T16:12:27Z
dc.date.issued 2014-02-21
dc.identifier.citation CARRIÓN, V. J., VAN DER VOORT, M., ARREBOLA, E., GUTIÉRREZ-BARRANQUERO, J. A., DE VICENTE, A., RAAIJMAKERS, J. M. & CAZORLA, F. M. 2014. Mangotoxin production of Pseudomonas syringae pv. syringae is regulated by MgoA. BMC Microbiology, 14:46, 1-13. http://dx.doi.org/10.1186/1471-2180-14-46 en
dc.identifier.volume 14 en
dc.identifier.startpage 1 en
dc.identifier.endpage 13 en
dc.identifier.issn 1471-2180
dc.identifier.uri http://hdl.handle.net/10468/2243
dc.identifier.doi 10.1186/1471-2180-14-46
dc.description.abstract Background: The antimetabolite mangotoxin is a key factor in virulence of Pseudomonas syringae pv. Syringae strains which cause apical necrosis of mango trees. Previous studies showed that mangotoxin biosynthesis is governed by the mbo operon. Random mutagenesis led to the identification of two other gene clusters that affect mangotoxin biosynthesis. These are the gacS/gacA genes and mgo operon which harbors the four genes mgoBCAD. Results: The current study shows that disruption of the nonribosomal peptide synthetase (NRPS) gene mgoA resulted in loss of mangotoxin production and reduced virulence on tomato leaves. Transcriptional analyses by qPCR and promoter reporter fusions revealed that mbo expression is regulated by both gacS/gacA and mgo genes. Also, expression of the mgo operon was shown to be regulated by gacS/gacA. Heterologous expression under the native promoter of the mbo operon resulted in mangotoxin production in non-producing P. syringae strains, but not in other Pseudomonas species. Also introduction of the mbo and mgo operons in nonproducing P. protegens Pf-5 did not confer mangotoxin production but did enhance transcription of the mbo promoter. Conclusions: From the data obtained in this study, we conclude that both mbo and mgo operons are under the control of the gacS/gacA two-component system and that the MgoA product acts as a positive regulator of mangotoxin biosynthesis. en
dc.description.sponsorship Junta de Andalucía (CICE), Spain (Ayudas Grupo PAIDI AGR-169; Proyecto de Excelencia P07-AGR-02471; fellowship to Víctor J. Carrión); Ministerio de Ciencia e Innovacion, Spain (Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica AGL2011-30354-C02-01); European Commission (FEDER; European Social Fund); University of Málaga, Spain (Plan Propio); Consejo Superior de Investigaciones Científicas, Spain (JAEDoc grant to Eva Arrebola) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher BioMed Central Ltd. en
dc.rights © Carrión et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://​creativecommons.​org/​publicdomain/​zero/​1.​0/​) applies to the data made available in this article, unless otherwise stated. en
dc.rights.uri http://​creativecommons.​org/​licenses/​by/​2.​0 en
dc.subject Plant-microbe interaction en
dc.subject Antimetabolite toxin en
dc.subject Mgo operon en
dc.subject GacS/GacA en
dc.title Mangotoxin production of Pseudomonas syringae pv. syringae is regulated by MgoA en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother José A. Gutiérrez-Barranquero, School of Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: jgutierrez@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Regional Government of Andalusia, Spain en
dc.contributor.funder Junta de Andalucía (CICE), Spain es
dc.contributor.funder Ministerio de Ciencia e Innovación, Spain es
dc.contributor.funder European Commission
dc.contributor.funder University of Málaga, Spain
dc.contributor.funder Consejo Superior de Investigaciones Científicas, Spain
dc.description.status Peer reviewed en
dc.identifier.journaltitle BMC Microbiology en
dc.internal.IRISemailaddress jgutierrez@ucc.ie en
dc.identifier.articleid 46


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© Carrión et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://​creativecommons.​org/​publicdomain/​zero/​1.​0/​) applies to the data made available in this article, unless otherwise stated. Except where otherwise noted, this item's license is described as © Carrión et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://​creativecommons.​org/​publicdomain/​zero/​1.​0/​) applies to the data made available in this article, unless otherwise stated.
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