Novel cyclic di-GMP effectors of the YajQ protein family control bacterial virulence

Show simple item record

dc.contributor.author An, Shi-Qi
dc.contributor.author Caly, Delphine L.
dc.contributor.author McCarthy, Yvonne
dc.contributor.author Murdoch, Sarah L.
dc.contributor.author Ward, Joseph
dc.contributor.author Febrer, Melanie
dc.contributor.author Dow, J. Maxwell
dc.contributor.author Ryan, Robert P.
dc.date.accessioned 2016-02-17T11:43:38Z
dc.date.available 2016-02-17T11:43:38Z
dc.date.issued 2014
dc.identifier.citation An S-q, Caly DL, McCarthy Y, Murdoch SL, Ward J, Febrer M, et al. (2014) Novel Cyclic di-GMP Effectors of the YajQ Protein Family Control Bacterial Virulence. PLoS Pathog 10(10): e1004429. doi:10.1371/journal.ppat.1004429
dc.identifier.volume 10 en
dc.identifier.issued 10 en
dc.identifier.issn 1553-7366
dc.identifier.uri http://hdl.handle.net/10468/2322
dc.identifier.doi 10.1371/journal.ppat.1004429
dc.description.abstract Bis-(3 ',5 ') cyclic di-guanylate (cyclic di-GMP) is a key bacterial second messenger that is implicated in the regulation of many critical processes that include motility, biofilm formation and virulence. Cyclic di-GMP influences diverse functions through interaction with a range of effectors. Our knowledge of these effectors and their different regulatory actions is far from complete, however. Here we have used an affinity pull-down assay using cyclic di-GMP-coupled magnetic beads to identify cyclic di-GMP binding proteins in the plant pathogen Xanthomonas campestris pv. campestris (Xcc). This analysis identified XC_3703, a protein of the YajQ family, as a potential cyclic di-GMP receptor. Isothermal titration calorimetry showed that the purified XC_3703 protein bound cyclic di-GMP with a high affinity (K-d similar to 2 mu M). Mutation of XC_3703 led to reduced virulence of Xcc to plants and alteration in biofilm formation. Yeast two-hybrid and far-western analyses showed that XC_3703 was able to interact with XC_2801, a transcription factor of the LysR family. Mutation of XC_2801 and XC_3703 had partially overlapping effects on the transcriptome of Xcc, and both affected virulence. Electromobility shift assays showed that XC_3703 positively affected the binding of XC_2801 to the promoters of target virulence genes, an effect that was reversed by cyclic di-GMP. Genetic and functional analysis of YajQ family members from the human pathogens Pseudomonas aeruginosa and Stenotrophomonas maltophilia showed that they also specifically bound cyclic di-GMP and contributed to virulence in model systems. The findings thus identify a new class of cyclic di-GMP effector that regulates bacterial virulence. en
dc.description.sponsorship Wellcome Trust, United Kingdom (WT093314MA; WT100204AIA) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Public Library of Science en
dc.rights © 2015 An et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited en
dc.rights.uri http://creativecommons.org/licenses/by/4.0/ en
dc.subject PilZ domain proteins en
dc.subject Campestris pv. campestris en
dc.subject Diffusible signal factor en
dc.subject Airway epithelial cells en
dc.subject Cystic fibrosis airway en
dc.subject Xanthomonas campestris en
dc.subject Pseudomonas aeruginosa en
dc.subject HD-GYP en
dc.subject Biofilm formation en
dc.subject 2nd messenger en
dc.title Novel cyclic di-GMP effectors of the YajQ protein family control bacterial virulence en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Max Dow, School of Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: m.dow@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.internal.wokid WOS:000344548800028
dc.contributor.funder Wellcome Trust, United Kingdom
dc.description.status Peer reviewed en
dc.identifier.journaltitle PLOS PATHOGENS en
dc.internal.IRISemailaddress m.dow@ucc.ie en
dc.identifier.articleid e1004429


Files in this item

This item appears in the following Collection(s)

Show simple item record

© 2015 An et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Except where otherwise noted, this item's license is described as © 2015 An et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
This website uses cookies. By using this website, you consent to the use of cookies in accordance with the UCC Privacy and Cookies Statement. For more information about cookies and how you can disable them, visit our Privacy and Cookies statement