Archaea appear to dominate the microbiome of Inflatella pellicula deep sea sponges

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dc.contributor.author Jackson, Stephen A.
dc.contributor.author Flemer, Burkhardt
dc.contributor.author McCann, Angela
dc.contributor.author Kennedy, Jonathan
dc.contributor.author Morrissey, John P.
dc.contributor.author O'Gara, Fergal
dc.contributor.author Dobson, Alan D. W.
dc.date.accessioned 2016-02-17T11:44:41Z
dc.date.available 2016-02-17T11:44:41Z
dc.date.issued 2013
dc.identifier.citation Jackson SA, Flemer B, McCann A, Kennedy J, Morrissey JP, O’Gara F, et al. (2013) Archaea Appear to Dominate the Microbiome of Inflatella pellicula Deep Sea Sponges. PLoS ONE 8(12): e84438. doi:10.1371/journal.pone.0084438
dc.identifier.volume 8 en
dc.identifier.issued 12 en
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10468/2346
dc.identifier.doi 10.1371/journal.pone.0084438
dc.description.abstract Microbes associated with marine sponges play significant roles in host physiology. Remarkable levels of microbial diversity have been observed in sponges worldwide through both culture-dependent and culture-independent studies. Most studies have focused on the structure of the bacterial communities in sponges and have involved sponges sampled from shallow waters. Here, we used pyrosequencing of 16S rRNA genes to compare the bacterial and archaeal communities associated with two individuals of the marine sponge Inflatella pellicula from the deep-sea, sampled from a depth of 2,900 m, a depth which far exceeds any previous sequence-based report of sponge-associated microbial communities. Sponge-microbial communities were also compared to the microbial community in the surrounding seawater. Sponge-associated microbial communities were dominated by archaeal sequencing reads with a single archaeal OTU, comprising similar to ∼60% and similar to ∼72% of sequences, being observed from Inflatella pellicula. Archaeal sequencing reads were less abundant in seawater (similar to ∼11% of sequences). Sponge-associated microbial communities were less diverse and less even than any other sponge-microbial community investigated to date with just 210 and 273 OTUs (97% sequence identity) identified in sponges, with 4 and 6 dominant OTUs comprising similar to ∼88% and similar to ∼89% of sequences, respectively. Members of the candidate phyla, SAR406, NC10 and ZB3 are reported here from sponges for the first time, increasing the number of bacterial phyla or candidate divisions associated with sponges to 43. A minor cohort from both sponge samples (similar to ∼0.2% and similar to ∼0.3% of sequences) were not classified to phylum level. A single OTU, common to both sponge individuals, dominates these unclassified reads and shares sequence homology with a sponge associated clone which itself has no known close relative and may represent a novel taxon. en
dc.description.sponsorship Department of Agriculture, Fisheries and Food (FIRM08/RDC/629); Marine Institute (Beaufort Marine Biodiscovery Research Award) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Public Library of Science en
dc.rights © 2015 Jackson et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited en
dc.rights.uri http://creativecommons.org/licenses/by/4.0/ en
dc.subject Ammonia-oxidizing archaea en
dc.subject Coral reef sponges en
dc.subject 16S ribosomal RNA en
dc.subject Marine sponges en
dc.subject Phylogenetic diversity en
dc.subject Cenarchaeum symbiosum en
dc.subject Antarctic sponges en
dc.subject Irish waters en
dc.subject Transmission en
dc.subject Communities en
dc.title Archaea appear to dominate the microbiome of Inflatella pellicula deep sea sponges en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Alan Dobson, Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: a.dobson@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.internal.rssid 251614995
dc.internal.wokid WOS:000329194700107
dc.contributor.funder Department of Agriculture, Fisheries and Food, Ireland
dc.contributor.funder Marine Institute
dc.description.status Peer reviewed en
dc.identifier.journaltitle PLOS ONE en
dc.internal.IRISemailaddress a.dobson@ucc.ie en
dc.identifier.articleid e84438


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© 2015 Jackson et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Except where otherwise noted, this item's license is described as © 2015 Jackson et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
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