Functional environmental screening of a metagenomic library identifies stlA; a unique salt tolerance locus from the human gut microbiome

Show simple item record

dc.contributor.author Culligan, Eamonn P.
dc.contributor.author Sleator, Roy D.
dc.contributor.author Marchesi, Julian R.
dc.contributor.author Hill, Colin
dc.date.accessioned 2016-02-17T11:44:41Z
dc.date.available 2016-02-17T11:44:41Z
dc.date.issued 2013
dc.identifier.citation Culligan EP, Sleator RD, Marchesi JR, Hill C (2013) Functional Environmental Screening of a Metagenomic Library Identifies stlA; A Unique Salt Tolerance Locus from the Human Gut Microbiome . PLoS ONE 8(12): e82985. doi:10.1371/journal.pone.0082985
dc.identifier.volume 8 en
dc.identifier.issued 12 en
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10468/2350
dc.identifier.doi 10.1371/journal.pone.0082985
dc.description.abstract Functional environmental screening of metagenomic libraries is a powerful means to identify and assign function to novel genes and their encoded proteins without any prior sequence knowledge. In the current study we describe the identification and subsequent analysis of a salt-tolerant clone from a human gut metagenomic library. Following transposon mutagenesis we identified an unknown gene (stlA, for "salt tolerance locus A") with no current known homologues in the databases. Subsequent cloning and expression in Escherichia coli MKH13 revealed that stlA confers a salt tolerance phenotype in its surrogate host. Furthermore, a detailed in silico analysis was also conducted to gain additional information on the properties of the encoded StlA protein. The stlA gene is rare when searched against human metagenome datasets such as MetaHit and the Human Microbiome Project and represents a novel and unique salt tolerance determinant which appears to be found exclusively in the human gut environment. en
dc.description.sponsorship Science Foundation Ireland (CSET Uplift Grant); European Society of Clinical Microbiology and Infectious Diseases, Switzerland (Research Fellowship) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Public Library of Science en
dc.rights © 2015 Culligan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited en
dc.rights.uri http://creativecommons.org/licenses/by/4.0/ en
dc.subject Outer membrane protein en
dc.subject Structure prediction en
dc.subject Osmotic stress en
dc.subject Benzodiazepine receptor en
dc.subject Akkermansia muciniphila en
dc.subject Gastrointestinal tract en
dc.subject Sequence analysis en
dc.subject Escherichia coli en
dc.subject Ankyrin repeat en
dc.subject I-TASSER en
dc.title Functional environmental screening of a metagenomic library identifies stlA; a unique salt tolerance locus from the human gut microbiome en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Colin Hill, Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: c.hill@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.internal.wokid WOS:000328731800087
dc.contributor.funder Science Foundation Ireland
dc.contributor.funder APC Microbiome Institute, College of Medicine and Health, University College Cork
dc.contributor.funder Royal Society, United Kingdom
dc.contributor.funder European Society of Clinical Microbiology and Infectious Diseases, Switzerland
dc.description.status Peer reviewed en
dc.identifier.journaltitle PLOS ONE en
dc.internal.IRISemailaddress c.hill@ucc.ie en
dc.identifier.articleid e82985


Files in this item

This item appears in the following Collection(s)

Show simple item record

© 2015 Culligan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Except where otherwise noted, this item's license is described as © 2015 Culligan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
This website uses cookies. By using this website, you consent to the use of cookies in accordance with the UCC Privacy and Cookies Statement. For more information about cookies and how you can disable them, visit our Privacy and Cookies statement