In silico assigned resistance genes confer Bifidobacterium with partial resistance to aminoglycosides but not to β-lactams

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dc.contributor.author Fouhy, Fiona
dc.contributor.author O'Connell Motherway, Mary
dc.contributor.author Fitzgerald, Gerald F.
dc.contributor.author Ross, R. Paul
dc.contributor.author Stanton, Catherine
dc.contributor.author van Sinderen, Douwe
dc.contributor.author Cotter, Paul D.
dc.date.accessioned 2016-02-17T11:44:42Z
dc.date.available 2016-02-17T11:44:42Z
dc.date.issued 2013
dc.identifier.citation Fouhy F, O’Connell Motherway M, Fitzgerald GF, Ross RP, Stanton C, van Sinderen D, et al. (2013) In Silico Assigned Resistance Genes Confer Bifidobacterium with Partial Resistance to Aminoglycosides but Not to Β-Lactams. PLoS ONE 8(12): e82653. doi:10.1371/journal.pone.0082653
dc.identifier.volume 8 en
dc.identifier.issued 12 en
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10468/2351
dc.identifier.doi 10.1371/journal.pone.0082653
dc.description.abstract Bifidobacteria have received significant attention due to their contribution to human gut health and the use of specific strains as probiotics. It is thus not surprising that there has also been significant interest with respect to their antibiotic resistance profile. Numerous culture-based studies have demonstrated that bifidobacteria are resistant to the majority of aminoglycosides, but are sensitive to β-lactams. However, limited research exists with respect to the genetic basis for the resistance of bifidobacteria to aminoglycosides. Here we performed an in-depth in silico analysis of putative Bifidobacterium-encoded aminoglycoside resistance proteins and β-lactamases and assess the contribution of these proteins to antibiotic resistance. The in silico-based screen detected putative aminoglycoside and β-lactam resistance proteins across the Bifidobacterium genus. Laboratory-based investigations of a number of representative bifidobacteria strains confirmed that despite containing putative β-lactamases, these strains were sensitive to β-lactams. In contrast, all strains were resistant to the aminoglycosides tested. To assess the contribution of genes encoding putative aminoglycoside resistance proteins in Bifidobacterium sp. two genes, namely Bbr_0651 and Bbr_1586, were targeted for insertional inactivation in B. breve UCC2003. As compared to the wild-type, the UCC2003 insertion mutant strains exhibited decreased resistance to gentamycin, kanamycin and streptomycin. This study highlights the associated risks of relying on the in silico assignment of gene function. Although several putative β-lactam resistance proteins are located in bifidobacteria, their presence does not coincide with resistance to these antibiotics. In contrast however, this approach has resulted in the identification of two loci that contribute to the aminoglycoside resistance of B. breve UCC2003 and, potentially, many other bifidobacteria. en
dc.description.sponsorship Irish Research Council for Science Engineering and Technology (EMBARK Scholarship); Teagasc (Walsh Fellowship); Science Foundation Ireland (Grant Nos.11/PI/1137; 07/CE/B1368; 02/CE/B124); Health Research Board (Postdoctoral Fellowship PDTM/20011/9) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Public Library of Science en
dc.rights © 2015 Fouhy et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited en
dc.rights.uri http://creativecommons.org/licenses/by/4.0/ en
dc.subject Susceptibility testing method en
dc.subject Bile salt hydrolase en
dc.subject Antimicrobial susceptibility en
dc.subject Antibiotic resistance en
dc.subject Beta-lactamases en
dc.subject Breve UCC2003 en
dc.subject Bacterial resistance en
dc.subject Functional characterization en
dc.subject Genome annotation en
dc.subject Strain Yakult en
dc.title In silico assigned resistance genes confer Bifidobacterium with partial resistance to aminoglycosides but not to β-lactams en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Gerald F. Fitzgerald, Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: g.fitzgerald@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.internal.wokid WOS:000328566700097
dc.contributor.funder Irish Research Council for Science Engineering and Technology
dc.contributor.funder Teagasc
dc.contributor.funder Science Foundation Ireland
dc.contributor.funder Health Research Board
dc.description.status Peer reviewed en
dc.identifier.journaltitle PLOS ONE en
dc.internal.IRISemailaddress g.fitzgerald@ucc.ie en
dc.identifier.articleid e82653


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© 2015 Fouhy et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Except where otherwise noted, this item's license is described as © 2015 Fouhy et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
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