Tn6188 - a novel transposon in Listeria monocytogenes responsible for tolerance to benzalkonium chloride

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dc.contributor.author Mueller, Anneliese
dc.contributor.author Rychli, Kathrin
dc.contributor.author Muhterem-Uyar, Meryem
dc.contributor.author Zaiser, Andreas
dc.contributor.author Stessl, Beatrix
dc.contributor.author Guinane, Caitriona M.
dc.contributor.author Cotter, Paul D.
dc.contributor.author Wagner, Martin
dc.contributor.author Schmitz-Esser, Stephan
dc.date.accessioned 2016-02-17T11:45:32Z
dc.date.available 2016-02-17T11:45:32Z
dc.date.issued 2013
dc.identifier.citation Müller A, Rychli K, Muhterem-Uyar M, Zaiser A, Stessl B, Guinane CM, et al. (2013) Tn6188 - A Novel Transposon in Listeria monocytogenes Responsible for Tolerance to Benzalkonium Chloride. PLoS ONE 8(10): e76835. doi:10.1371/journal.pone.0076835 en
dc.identifier.volume 8 en
dc.identifier.issued 10 en
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10468/2364
dc.identifier.doi 10.1371/journal.pone.0076835
dc.description.abstract Controlling the food-borne pathogen Listeria (L.) monocytogenes is of great importance from a food safety perspective, and thus for human health. The consequences of failures in this regard have been exemplified by recent large listeriosis outbreaks in the USA and Europe. It is thus particularly notable that tolerance to quaternary ammonium compounds such as benzalkonium chloride (BC) has been observed in many L. monocytogenes strains. However, the molecular determinants and mechanisms of BC tolerance of L. monocytogenes are still largely unknown. Here we describe Tn6188, a novel transposon in L. monocytogenes conferring tolerance to BC. Tn6188 is related to Tn554 from Staphylococcus (S.) aureus and other Tn554-like transposons such as Tn558, Tn559 and Tn5406 found in various Firmicutes. Tn6188 comprises 5117 bp, is integrated chromosomally within the radC gene and consists of three transposase genes (tnpABC) as well as genes encoding a putative transcriptional regulator and QacH, a small multidrug resistance protein family (SMR) transporter putatively associated with export of BC that shows high amino acid identity to Smr/QacC from S. aureus and to EmrE from Escherichia coli. We screened 91 L. monocytogenes strains for the presence of Tn6188 by PCR and found Tn6188 in 10 of the analyzed strains. These isolates were from food and food processing environments and predominantly from serovar 1/2a. L. monocytogenes strains harboring Tn6188 had significantly higher BC minimum inhibitory concentrations (MICs) (28.5 +/- 4.7 mg/l) than strains without Tn6188 (14 +/- 3.2 mg/l). Using quantitative reverse transcriptase PCR we could show a significant increase in qacH expression in the presence of BC. QacH deletion mutants were generated in two L. monocytogenes strains and growth analysis revealed that Delta qacH strains had lower BC MICs than wildtype strains. In conclusion, our results provide evidence that Tn6188 is responsible for BC tolerance in various L. monocytogenes strains. en
dc.description.sponsorship Austrian Science Fund (FWF grant no. P22703‐B17); European Commission EU BIOTRACER (contract no. 036272), EU grant FP7‐KBBE‐2010‐4 (FOODSEG)) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Public Library of Science en
dc.rights © 2013 Müller et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited en
dc.rights.uri http://creativecommons.org/licenses/by/4.0/ en
dc.subject Quaternary ammonium compounds en
dc.subject Plasmid-borne cadmium en
dc.subject Staphylococcus aureus en
dc.subject Multidrug resistance en
dc.subject Processing environment en
dc.subject Comparative genomics en
dc.subject Escherichia coli en
dc.subject Gene en
dc.subject Outbreak en
dc.subject Disinfectants en
dc.title Tn6188 - a novel transposon in Listeria monocytogenes responsible for tolerance to benzalkonium chloride en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Paul D. Cotter, Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland. +353-21-490-3000 en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.internal.wokid WOS:000325434500088
dc.contributor.funder Austrian Science Fund en
dc.contributor.funder European Commission en
dc.description.status Peer reviewed en
dc.identifier.journaltitle PLOS ONE en
dc.internal.IRISemailaddress P.Cotter@ucc.ie en
dc.identifier.articleid e76835


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© 2013 Müller et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Except where otherwise noted, this item's license is described as © 2013 Müller et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
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