The HD-GYP Domain Protein RpfG of Xanthomonas oryzae pv. oryzicola Regulates Synthesis of Extracellular Polysaccharides that Contribute to Biofilm Formation and Virulence on Rice

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dc.contributor.author Zhang, Yuanbao
dc.contributor.author Wei, Chao
dc.contributor.author Jiang, Wendi
dc.contributor.author Wang, Lei
dc.contributor.author Li, Churui
dc.contributor.author Wang, Yunyue
dc.contributor.author Dow, J. Maxwell
dc.contributor.author Sun, Wenxian
dc.date.accessioned 2016-02-17T11:46:19Z
dc.date.available 2016-02-17T11:46:19Z
dc.date.issued 2013
dc.identifier.citation Zhang Y, Wei C, Jiang W, Wang L, Li C, Wang Y, et al. (2013) The HD-GYP Domain Protein RpfG of Xanthomonas oryzae pv. oryzicola Regulates Synthesis of Extracellular Polysaccharides that Contribute to Biofilm Formation and Virulence on Rice. PLoS ONE 8(3): e59428. doi:10.1371/journal.pone.0059428 en
dc.identifier.volume 8 en
dc.identifier.issued 3 en
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10468/2386
dc.identifier.doi 10.1371/journal.pone.0059428
dc.description.abstract Bacterial leaf streak caused by Xanthomonas oryzae pv. oryzicola (Xoc) is one of the most important diseases in rice. However, little is known about the pathogenicity mechanisms of Xoc. Here we have investigated the function of three HD-GYP domain regulatory proteins in biofilm formation, the synthesis of virulence factors and virulence of Xoc. Deletion of rpfG resulted in altered production of extracellular polysaccharides (EPS), abolished virulence on rice and enhanced biofilm formation, but had little effect on the secretion of proteases and motility. In contrast, mutational analysis showed that the other two HD-GYP domain proteins had no effect on virulence factor synthesis and tested phenotypes. Mutation of rpfG led to up-regulation of the type III secretion system and altered expression of three putative glycosyltransferase genes gumD, pgaC and xagB, which are part of operons directing the synthesis of different extracellular polysaccharides. The pgaABCD and xagABCD operons were greatly up-regulated in the Xoc Delta rpfG mutant, whereas the expression of the gum genes was unaltered or slightly enhanced. The elevated biofilm formation of the Xoc Delta rpfG mutant was dramatically reduced upon deletion of gumD, xagA and xagB, but not when pgaA and pgaC were deleted. Interestingly, only the Delta gumD mutant, among these single gene mutants, exhibits multiple phenotype alterations including reduced biofilm and EPS production and attenuated virulence on rice. These data indicate that RpfG is a global regulator that controls biofilm formation, EPS production and bacterial virulence in Xoc and that both gumD- and xagB-dependent EPS contribute to biofilm formation under different conditions. en
dc.description.sponsorship 973 program (2011CB100700); National Natural Science Foundation of China (NSFC) (31272007); PCSIRT Program (IRT1042, NCET-09-0736); Ph.D. Programs Foundation of Ministry of Education of China en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Public Library of Science en
dc.rights © 2013 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited en
dc.rights.uri http://creativecommons.org/licenses/by/4.0/ en
dc.subject Diffusible Signal Factor en
dc.subject Di-Gmp Turnover en
dc.subject Pseudomonas-Aeruginosa en
dc.subject 2-Component System en
dc.subject Gene-Expression en
dc.subject Campestris en
dc.subject Vesicatoria en
dc.subject Phosphodiesterase en
dc.subject Pathogenicity en
dc.subject Sequences en
dc.title The HD-GYP Domain Protein RpfG of Xanthomonas oryzae pv. oryzicola Regulates Synthesis of Extracellular Polysaccharides that Contribute to Biofilm Formation and Virulence on Rice en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother J. Maxwell Dow, Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: m.dow@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.internal.wokid WOS:000317480700121
dc.description.status Peer reviewed en
dc.identifier.journaltitle PLOS ONE en
dc.internal.IRISemailaddress m.dow@ucc.ie en
dc.identifier.articleid e59428


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© 2013 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Except where otherwise noted, this item's license is described as © 2013 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
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