In vivo activity of Nisin A and Nisin V against Listeria monocytogenes in mice

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dc.contributor.author Campion, Alicia M.
dc.contributor.author Casey, Pat G.
dc.contributor.author Field, Des
dc.contributor.author Cotter, Paul D.
dc.contributor.author Hill, Colin
dc.contributor.author Ross, R. Paul
dc.date.accessioned 2016-02-25T17:15:42Z
dc.date.available 2016-02-25T17:15:42Z
dc.date.issued 2013-02-01
dc.identifier.citation CAMPION, A., CASEY, P. G., FIELD, D., COTTER, P. D., HILL, C. & ROSS, R. P. 2013. In vivo activity of Nisin A and Nisin V against Listeria monocytogenes in mice. BMC Microbiology, 13:23, 1-8. http://dx.doi.org/10.1186/1471-2180-13-23 en
dc.identifier.volume 13 en
dc.identifier.startpage 1 en
dc.identifier.endpage 8 en
dc.identifier.issn 1471-2180
dc.identifier.uri http://hdl.handle.net/10468/2401
dc.identifier.doi 10.1186/1471-2180-13-23
dc.description.abstract Background: Lantibiotics are post-translationally modified antimicrobial peptides, of which nisin A is the most extensively studied example. Bioengineering of nisin A has resulted in the generation of derivatives with increased in vitro potency against Gram-positive bacteria. Of these, nisin V (containing a Met21Val change) is noteworthy by virtue of exhibiting enhanced antimicrobial efficacy against a wide range of clinical and food-borne pathogens, including Listeria monocytogenes. However, this increased potency has not been tested in vivo. Results: Here we address this issue by assessing the ability of nisin A and nisin V to control a bioluminescent strain of Listeria monocytogenes EGDe in a murine infection model. More specifically, Balb/c mice were infected via the intraperitoneal route at a dose of 1 × 105 cfu/animal and subsequently treated intraperitoneally with either nisin V, nisin A or a PBS control. Bioimaging of the mice was carried out on day 3 of the trial. Animals were then sacrificed and levels of infection were quantified in the liver and spleen. Conclusion: This analysis revealed that nisin V was more effective than Nisin A with respect to controlling infection and therefore merits further investigation with a view to potential chemotherapeutic applications. en
dc.description.sponsorship Higher Education Authority (Programme for Research in Third-Level Institutions (PRTLI) PhD Programme in Molecular Cell Biology awarded to Alicia Campion); Irish Government (National Development Plan); Science Foundation Ireland (Centre for Science, Engineering and Technology (SFI-CSET) Principal Investigator funding to Paul D. Cotter, Colin Hill and R. Paul Ross) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher BioMed Central Ltd. en
dc.rights © Campion et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. en
dc.rights.uri http://​creativecommons.​org/​licenses/​by/​2.​0 en
dc.subject Antimicrobial en
dc.subject Lantibiotic en
dc.subject Bacteriocin en
dc.subject Peptide engineering en
dc.subject Mutagenesis en
dc.subject Nisin en
dc.title In vivo activity of Nisin A and Nisin V against Listeria monocytogenes in mice en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Colin Hill, School of Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: c.hill@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Enterprise Ireland en
dc.contributor.funder Higher Education Authority en
dc.contributor.funder Irish Research Council for Science Engineering and Technology en
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder Irish Government
dc.description.status Peer reviewed en
dc.identifier.journaltitle BMC Microbiology en
dc.internal.IRISemailaddress c.hill@ucc.ie en
dc.identifier.articleid 23


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© Campion et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Except where otherwise noted, this item's license is described as © Campion et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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