Influence of internalin a murinisation on host resistance to orally acquired listeriosis in mice

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dc.contributor.author Bergmann, Silke
dc.contributor.author Beard, Philippa M.
dc.contributor.author Pasche, Bastian
dc.contributor.author Lienenklaus, Stefan
dc.contributor.author Weiss, Siegfried
dc.contributor.author Gahan, Cormac G. en
dc.contributor.author Schughart, Klaus
dc.contributor.author Lengeling, Andreas
dc.date.accessioned 2016-02-25T17:20:02Z
dc.date.available 2016-02-25T17:20:02Z
dc.date.issued 2013-04-23
dc.identifier.citation BERGMANN, S., BEARD, P. M., PASCHE, B., LIENENKLAUS, S., WEISS, S., GAHAN, C. G. M., SCHUGHART, K. & LENGELING, A. 2013. Influence of internalin a murinisation on host resistance to orally acquired listeriosis in mice. BMC Microbiology, 13:90, 1-16. http://dx.doi.org/10.1186/1471-2180-13-90 en
dc.identifier.volume 13 en
dc.identifier.startpage 1 en
dc.identifier.endpage 16 en
dc.identifier.issn 1471-2180
dc.identifier.uri http://hdl.handle.net/10468/2403
dc.identifier.doi 10.1186/1471-2180-13-90
dc.description.abstract Background: The bacterial surface protein internalin (InlA) is a major virulence factor of the food-born pathogen Listeria monocytogenes. It plays a critical role in the bacteria crossing the host intestinal barrier by a species-specific interaction with the cell adhesion molecule E-cadherin. In mice, the interaction of InlA with murine E-cadherin is impaired due to sequence-specific binding incompatibilities. We have previously used the approach of ‘murinisation’ to establish an oral listeriosis infection model in mice by exchanging two amino acid residues in InlA. This dramatically increases binding to mouse E-cadherin. In the present study, we have used bioluminescent murinised and non-murinised Listeria strains to examine the spatiotemporal dissemination of Listeria in four diverse mouse genetic backgrounds after oral inoculation. Results: The murinised Listeria monocytogenes strain showed enhanced invasiveness and induced more severe infections in all four investigated mouse inbred strains compared to the non-murinised Listeria strain. We identified C57BL/6J mice as being most resistant to orally acquired listeriosis whereas C3HeB/FeJ, A/J and BALB/cJ mice were found to be most susceptible to infection. This was reflected in faster kinetics of Listeria dissemination, higher bacterial loads in internal organs, and elevated serum levels of IL-6, IFN-γ, TNF-α and CCL2 in the susceptible strains as compared to the resistant C57BL/6J strain. Importantly, murinisation of InlA did not cause enhanced invasion of Listeria monocytogenes into the brain. Conclusion: Murinised Listeria are able to efficiently cross the intestinal barrier in mice from diverse genetic backgrounds. However, expression of murinized InlA does not enhance listerial brain invasion suggesting that crossing of the blood brain barrier and crossing of the intestinal epithelium are achieved by Listeria monocytogenes through different molecular mechanisms. en
dc.description.sponsorship National German Genome Network (NGFN-Plus Grant Number 01GS0855); European Commission (EUMODIC Project - Framework Programme 6: LSHG-CT-2006-037188; European COST Action ‘SYSGENET’ BM901); Biotechnology and Biological Sciences Research Council, United Kingdom (Institute Strategic Grant); Helmholtz Centre for Infection Research (HZI), Germany (Institute Strategic Grant) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher BioMed Central Ltd. en
dc.rights © Bergmann et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. en
dc.rights.uri http://​creativecommons.​org/​licenses/​by/​2.​0 en
dc.subject Central nervous system en
dc.subject I interferon receptor en
dc.subject Monocytogenes infection en
dc.subject E-cadherin en
dc.subject Genetic control en
dc.subject Salmonella Typhimurium en
dc.subject Bacterial infection en
dc.subject Intestinal barrier en
dc.subject Surface protein en
dc.subject Innate immunity en
dc.title Influence of internalin a murinisation on host resistance to orally acquired listeriosis in mice en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Cormac Gahan, School of Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: C.Gahan@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder National German Genome Network
dc.contributor.funder European Commission
dc.contributor.funder Biotechnology and Biological Sciences Research Council
dc.contributor.funder Helmholtz Centre for Infection Research, Germany
dc.description.status Peer reviewed en
dc.identifier.journaltitle BMC Microbiology en
dc.internal.IRISemailaddress c.gahan@ucc.ie en
dc.identifier.articleid 90


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© Bergmann et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Except where otherwise noted, this item's license is described as © Bergmann et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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