Laparotomy and laparoscopy diversely affect macrophage-associated antimicrobial activity in a murine model

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dc.contributor.author Huang, Shungen
dc.contributor.author Li, Yi Ping
dc.contributor.author Zhang, Qi
dc.contributor.author Redmond, H. Paul
dc.contributor.author Wang, Jiang Huai
dc.contributor.author Wang, Jian
dc.date.accessioned 2016-02-25T17:21:53Z
dc.date.available 2016-02-25T17:21:53Z
dc.date.issued 2013-06-20
dc.identifier.citation HUANG, S. G., LI, Y. P., ZHANG, Q., REDMOND, H. P., WANG, J. H. & WANG, J. 2013. Laparotomy and laparoscopy diversely affect macrophage-associated antimicrobial activity in a murine model. BMC Immunology, 14:27, 1-8. http://dx.doi.org/10.1186/1471-2172-14-27 en
dc.identifier.volume 14 en
dc.identifier.startpage 1 en
dc.identifier.endpage 8 en
dc.identifier.issn 1471-2172
dc.identifier.uri http://hdl.handle.net/10468/2404
dc.identifier.doi 10.1186/1471-2172-14-27
dc.description.abstract Background: Surgical intervention-related trauma contributes largely to the development of postoperative immunosuppression, with reduced resistance to secondary bacterial infection. This study compared the impact of laparotomy versus laparoscopy on macrophage-associated bactericidal ability and examined whether laparotomy renders the host more susceptible to microbial infection. Results: BALB/c mice were randomized into control, laparotomy, and laparoscopy groups. Laparotomy, but not laparoscopy, significantly downregulated CR3 expression on macrophages, diminished macrophage-induced uptake and phagocytosis of E. coli and S. aureus, and impaired macrophage-mediated intracellular bacterial killing. Consistent with this, mice that underwent laparotomy displayed substantially higher bacterial counts in the blood and visceral organs as well as a significantly enhanced mortality rate following bacterial infection, whereas mice subjected to laparoscopy did not show any defects in their bacterial clearance. Conclusion: Laparotomy has an adverse effect on host innate immunity against microbial infection by impairing macrophage-mediated phagocytosis and killing of the invaded bacteria. By contrast, laparoscopy appears to preserve macrophage-associated bactericidal ability, thus alleviating the development of postoperative immunosuppression. en
dc.description.sponsorship Natural Science Foundation of Jiangsu Province, China (BK2011310); Innovative Team of Jiangsu Province, China (LJ201141); Jiangsu Province Program of Innovative and Entrepreneurial Talents, China (2011–2014) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher BioMed Central Ltd. en
dc.rights © Huang et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. en
dc.rights.uri http://​creativecommons.​org/​licenses/​by/​2.​0 en
dc.subject Laparotomy en
dc.subject Laparoscopy en
dc.subject Phagocytosis en
dc.subject Bactericidal activity en
dc.subject Innate immunity en
dc.subject Macrophages en
dc.title Laparotomy and laparoscopy diversely affect macrophage-associated antimicrobial activity in a murine model en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Henry Paul Redmond, Department of Surgery, University College Cork, Cork, Ireland. +353-21-490-3000 Email: henry.redmond@hse.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Natural Science Foundation of Jiangsu Province, China
dc.contributor.funder Innovative Team of Jiangsu Province, China
dc.contributor.funder Jiangsu Province Program of Innovative and Entrepreneurial Talents, China
dc.description.status Peer reviewed en
dc.identifier.journaltitle BMC Immunology en
dc.internal.IRISemailaddress henry.redmond@hse.ie en
dc.identifier.articleid 27


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© Huang et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Except where otherwise noted, this item's license is described as © Huang et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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