The role of iron uptake in pathogenicity and symbiosis in Photorhabdus luminescens TT01

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dc.contributor.author Watson, Robert J.
dc.contributor.author Millichap, Peter
dc.contributor.author Joyce, Susan A.
dc.contributor.author Reynolds, Stuart
dc.contributor.author Clarke, David J.
dc.date.accessioned 2011-03-14T09:48:59Z
dc.date.available 2011-03-14T09:48:59Z
dc.date.issued 2010-06-22
dc.identifier.citation Watson, R.J., Millichap, P., Joyce, S.A., Reynolds, S., Clarke, D.J., 2010. The role of iron uptake in pathogenicity and symbiosis in Photorhabdus luminescens TT01. BMC Microbiology, 10, 177 en
dc.identifier.volume 10 en
dc.identifier.startpage 177 en
dc.identifier.issn 1471-2180
dc.identifier.uri http://hdl.handle.net/10468/243
dc.identifier.doi 10.1186/1471-2180-10-177
dc.description.abstract Background Photorhabdus are Gram negative bacteria that are pathogenic to insect larvae whilst also having a mutualistic interaction with nematodes from the family Heterorhabditis. Iron is an essential nutrient and bacteria have different mechanisms for obtaining both the ferrous (Fe2+) and ferric (Fe3+) forms of this metal from their environments. In this study we were interested in analyzing the role of Fe3+ and Fe2+ iron uptake systems in the ability of Photorhabdus to interact with its invertebrate hosts. Results We constructed targeted deletion mutants of exbD, feoABC and yfeABCD in P. luminescens TT01. The exbD mutant was predicted to be crippled in its ability to obtain Fe3+ and we show that this mutant does not grow well in iron-limited media. We also show that this mutant was avirulent to the insect but was unaffected in its symbiotic interaction with Heterorhabditis. Furthermore we show that a mutation in feoABC (encoding a predicted Fe2+ permease) was unaffected in both virulence and symbiosis whilst the divalent cation transporter encoded by yfeABCD is required for virulence in the Tobacco Hornworm, Manduca sexta (Lepidoptera) but not in the Greater Wax Moth, Galleria mellonella (Lepidoptera). Moreover the Yfe transporter also appears to have a role during colonization of the IJ stage of the nematode. Conclusion In this study we show that iron uptake (via the TonB complex and the Yfe transporter) is important for the virulence of P. luminescens to insect larvae. Moreover this study also reveals that the Yfe transporter appears to be involved in Mn2+-uptake during growth in the gut lumen of the IJ nematode. Therefore, the Yfe transporter in P. luminescens TT01 is important during colonization of both the insect and nematode and, moreover, the metal ion transported by this pathway is host-dependent. en
dc.description.sponsorship Biotechnology and Biological Sciences Research Council, United Kingdom (Studentships); Biotechnology and Biological Sciences Research Council, United Kingdom (86/EGA16183) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher BioMed Central Ltd. en
dc.relation.uri http://www.biomedcentral.com/1471-2180/10/177
dc.rights © 2010 Watson et al; licensee BioMed Central Ltd. en
dc.rights.uri http://www.biomedcentral.com/info/about/license en
dc.subject Photorhabdus en
dc.subject Iron uptake systems en
dc.subject.lcsh Pathogenic bacteria en
dc.subject.lcsh Nematodes en
dc.subject.lcsh Enterobacteriaceae -- Molecular aspects en
dc.title The role of iron uptake in pathogenicity and symbiosis in Photorhabdus luminescens TT01 en
dc.type Article (peer-reviewed) en
dc.internal.authorurl http://publish.ucc.ie/researchprofiles/D010/davidclarke/home en
dc.internal.authorcontactother David Clarke, Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: david.clarke@ucc.ie en
dc.internal.authorcontactother Susan Joyce, Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: s.joyce@ucc.ie en
dc.internal.availability Full text available en
dc.date.updated 2011-03-10T16:26:24Z
dc.description.version Published Version en
dc.internal.rssid 59235809
dc.internal.rssid 424083756
dc.contributor.funder Biotechnology and Biological Sciences Research Council en
dc.contributor.funder Science Foundation Ireland en
dc.description.status Peer reviewed en
dc.identifier.journaltitle BMC Microbiology en
dc.internal.copyrightchecked RoMEO en
dc.internal.IRISemailaddress david.clarke@ucc.ie en


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