Immunostimulatory effects of different aspects of aquaculture on the host response in the edible sea urchin, paracentrotus lividus

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dc.contributor.advisor Culloty, Sarah C. en
dc.contributor.advisor Burnell, Gavin en Cipriano-Maack, Ashley N. 2016-05-12T09:12:40Z 2016-05-12T09:12:40Z 2016 2016
dc.identifier.citation Cipriano-Maack, A. N. 2016. Immunostimulatory effects of different aspects of aquaculture on the host response in the edible sea urchin, paracentrotus lividus. PhD Thesis, University College Cork. en
dc.identifier.endpage 192 en
dc.description.abstract Aquaculture is a fast-growing industry contributing to global food security and sustainable aquaculture, which may reduce pressures on capture fisheries. The overall objective of this thesis was to look at the immunostimulatory effects of different aspects of aquaculture on the host response of the edible sea urchin, Paracentrotus lividus, which are a prized delicacy (roe) in many Asian and Mediterranean countries. In Chapter 1, the importance of understanding the biology, ecology, and physiology of P. lividus, as well as the current status in the culture of this organism for mass production and introducing the thesis objectives for following chapters is discussed. As the research commenced, the difficulties of identifying individuals for repeat sampling became clear; therefore, Chapter 2 was a tagging experiment that indicated PIT tagging was a successful way of identifying individual sea urchins over time with a high tag retention rate. However, it was also found that repeat sampling via syringe to measure host response of an individual caused stress which masked results and thus animals would be sampled and sacrificed going forward. Additionally, from personal observations and discussion with peers, it was suggested to look at the effect that diet has on sea urchin immune function and the parameters I measured which led to Chapter 3. In this chapter, both Laminaria digitata and Mytilus edulis were shown to influence measured immune parameters of differential cell counts, nitric oxide production, and lysozyme activity. Therefore, trials commencing after Trial 5 in Chapter 4, were modified to include starvation in order to remove any effect of diet. Another important aspect of culturing any organism is the study of their immune function and its response to several immunostimulatory agents (Chapter 4). Zymosan A was shown to be an effective immunostimulatory agent in P. lividus. Further work on handled/stored animals (Chapter 5) showed Zymosan A reduced the measured levels of some immune parameters measured relative to the control, which may reduce the amount of stress in the animals. In Chapter 6, animals were infected with Vibrio anguillarum and, although V. anguillarum, impacted immune parameters of P. lividus, it did not cause mortality as predicted. Lastly, throughout this thesis work, it was noted that the immune parameters measured produced different values at different times of the year (Chapter 7); therefore, using collated baseline (control) data, results were compiled to observe seasonal effects. It was determined that both seasonality and sourcing sites influenced immune parameter measurements taken at different times throughout the year. In conclusion, this thesis work fits into the framework of development of aquaculture practices that affect immune function of the host and future research focusing on the edible sea urchin, P. lividus. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher University College Cork en
dc.rights © 2016, Ashley N. Cipriano-Maack. en
dc.rights.uri en
dc.subject Sea urchins en
dc.subject Paracentrotus lividus en
dc.subject Immunostimulants en
dc.subject Zymosan A en
dc.subject Handling and simulated storage en
dc.subject Seasonality and site en
dc.subject PIT tagging en
dc.subject Laminaria digitata en
dc.subject Vibrio anguillarum en
dc.subject Lysozyme activity en
dc.subject Nitric oxide en
dc.subject Cell viability en
dc.subject Differential cell counts en
dc.title Immunostimulatory effects of different aspects of aquaculture on the host response in the edible sea urchin, paracentrotus lividus en
dc.type Doctoral thesis en
dc.type.qualificationlevel Doctoral en
dc.type.qualificationname PhD (Science) en
dc.internal.availability Full text available en No embargo required en
dc.description.version Accepted Version
dc.contributor.funder The Thomas Crawford-Hayes Fellowship, Plymouth University en
dc.description.status Not peer reviewed en Biological, Earth & Environmental Sciences en
dc.check.type No Embargo Required
dc.check.reason No embargo required en
dc.check.opt-out No en
dc.thesis.opt-out false
dc.check.embargoformat Not applicable en
dc.internal.conferring Summer 2016 en

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© 2016, Ashley N. Cipriano-Maack. Except where otherwise noted, this item's license is described as © 2016, Ashley N. Cipriano-Maack.
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