Evaluation of a gelatin modified poly(ɛ-caprolactone) film as a scaffold for lung disease

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dc.contributor.author Kosmala, Aleksandra
dc.contributor.author Fitzgerald, Michelle
dc.contributor.author Moore, Eric J.
dc.contributor.author Stam, Frank
dc.date.accessioned 2016-05-16T13:29:02Z
dc.date.available 2016-05-16T13:29:02Z
dc.date.copyright 2017-04-19
dc.date.issued 2016-04-19
dc.identifier.citation Kosmala, A., Fitzgerald, M., Moore, E. and Stam, F. (2016) 'Evaluation of a Gelatin Modified Poly(ɛ-Caprolactone) Film as a Scaffold for Lung Disease', Analytical Letters, 50(1), pp. 219-232. doi: 10.1080/00032719.2016.1163363 en
dc.identifier.volume 50
dc.identifier.issued 1
dc.identifier.startpage 219
dc.identifier.endpage 232
dc.identifier.issn 0003-2719
dc.identifier.other 1532-236X
dc.identifier.uri http://hdl.handle.net/10468/2580
dc.identifier.doi 10.1080/00032719.2016.1163363
dc.description.abstract Lung transplantation is a necessary step for the patients with the end-stage of chronic obstructive pulmonary disease. The use of artificial lungs is a promising alternative to natural lung transplantation which is complicated and is restricted by low organ donations. For successful lung engineering, it is important to choose the correct combination of specific biological cells and a synthetic carrier polymer. The focus of this study was to investigate the interactions of human lung epithelial cell line NCl-H292 that is involved in lung tissue development with the biodegradable poly(ϵ-caprolactone) before and after its chemical modification to evaluate potential for use in artificial lung formation. Also, the effect of polymer chemical modification on its mechanical and surface properties has been investigated. The poly(ϵ-caprolactone) surface was modified using aminolysis followed by immobilization of gelatine. The unmodified and modified polymer surfaces were characterized for roughness, tensile strength, and NCl-H292 metabolic cell activity. The results showed for the first time the possibility for NCI-H292 cells to adhere on this polymeric material. The Resazurin assay showed that the metabolic activity at 24 hours post seeding of 80% in the presence of the unmodified and greater than 100% in the presence of the modified polymer was observed. The roughness of the poly(ϵ-caprolactone) increased from 4 nm to 26 nm and the film strength increased from 0.01 kN to 0.045 kN when the material was chemically modified. The results obtained to date show potential for using modified poly(ϵ-caprolactone) as a scaffold for lung tissue engineering. en
dc.description.sponsorship European Commission (EU Seventh Framework Programme FP7/2007-2013 under grant agreement no. 258909.) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Taylor & Francis en
dc.rights © 2016 Taylor & Francis en
dc.subject Tissue engineering, en
dc.subject Viability en
dc.subject Biopolymers en
dc.subject Immobilization en
dc.subject Tissue cell culture en
dc.title Evaluation of a gelatin modified poly(ɛ-caprolactone) film as a scaffold for lung disease en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Frank Stam, Tyndall Microsystems, University College Cork, Cork, Ireland. +353-21-490-3000 Email: frank.stam@tyndall.ie en
dc.internal.availability Full text available en
dc.check.info Access to this item is restricted until 12 months after publication by the request of the publisher. en
dc.check.date 2017-04-19
dc.date.updated 2016-05-12T19:40:08Z
dc.description.version Accepted Version en
dc.internal.rssid 347879713
dc.description.status Peer reviewed en
dc.identifier.journaltitle Analytical Letters en
dc.internal.copyrightchecked Yes. !!CORA!! Yes en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress frank.stam@tyndall.ie en


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