Nanofabrication towards biophotonics

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dc.contributor.advisor O'Riordan, Alan en
dc.contributor.advisor Lovera, Pierre en Jones, Daniel 2016-06-10T10:53:41Z 2016-06-10T10:53:41Z 2015 2015
dc.identifier.citation Jones, D. 2015. Nanofabrication towards biophotonics. PhD Thesis, University College Cork. en
dc.identifier.endpage 145 en
dc.description.abstract This thesis explores methods for fabrication of nanohole arrays, and their integration into a benchtop system for use as sensors or anti-counterfeit labels. Chapter 1 gives an introduction to plasmonics and more specifically nanohole arrays and how they have potential as label free sensors compared to the current biosensors on the market. Various fabrication methods are explored, including Focused Ion Beam, Electron Beam Lithography, Nanoimprint lithography, Template stripping and Phase Shift Lithography. Focused Ion Beam was chosen to fabricate the nanohole arrays due to its suitability for rapid prototyping and it’s relatively low cost. In chapter 2 the fabrication of nanohole arrays using FIB is described, and the samples characterised. The fabricated nanohole arrays are tested as bulk refractive index sensors, before a bioassay using whole molecule human IgG antibodies and antigen is developed and performed on the senor. In chapter 3 the fabricated sensors are integrated into a custom built system, capable of real time, multiplexed detection of biomolecules. Here, scFv antibodies of two biomolecules relevant to the detection of pancreatic cancer (C1q and C3) are attached to the nanohole arrays, and detection of their complementary proteins is demonstrated both in buffer (10 nM detection of C1q Ag) and human serum. Chapter 4 explores arrays of anisotropic (elliptical) nanoholes and shows how the shape anisotropy induces polarisation sensitive transmission spectra, in both simulations and fabricated arrays. The potential use of such samples as visible and NIR tag for anti-counterfeiting applications is demonstrated. Finally, chapter 5 gives a summary of the work completed and discusses potential future work in this area. en
dc.description.sponsorship European Union (EU Framework 7 under the Phast-ID project [258238]); Science Foundation Ireland (EU under US-Ireland Agri-Sense project [12/US/I2476]); Higher Education Authority (PRTLI programs [Cycle 3 Nanoscience and Cycle 4 INSPIRE]) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher University College Cork en
dc.rights © 2015, Daniel Jones. en
dc.rights.uri en
dc.subject Immuno-sensor en
dc.subject Plasmonics en
dc.subject Nanohole array en
dc.subject Real time label-free biosensing en
dc.subject C1q & C3 antigen detection en
dc.subject Pancreatic cancer biomarkers en
dc.title Nanofabrication towards biophotonics en
dc.type Doctoral thesis en
dc.type.qualificationlevel Doctoral en
dc.type.qualificationname PHD (Engineering) en
dc.internal.availability Full text available en No embargo required en
dc.description.version Accepted Version
dc.contributor.funder European Union en
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder Higher Education Authority en
dc.description.status Not peer reviewed en Electrical and Electronic Engineering en Tyndall National Institute en
dc.check.type No Embargo Required
dc.check.reason No embargo required en
dc.check.opt-out Not applicable en
dc.thesis.opt-out false
dc.check.embargoformat Not applicable en
dc.internal.conferring Summer 2015 en

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