Interaction between Candida albicans and Pseudomonas aeruginosa

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dc.contributor.advisor Morrissey, John P. en
dc.contributor.author Konstantinidou, Nina
dc.date.accessioned 2016-08-22T11:47:10Z
dc.date.available 2016-08-22T11:47:10Z
dc.date.issued 2016
dc.date.submitted 2016
dc.identifier.citation Konstantinidou, N. 2016. Interaction between Candida albicans and Pseudomonas aeruginosa. PhD Thesis, University College Cork. en
dc.identifier.endpage 150 en
dc.identifier.uri http://hdl.handle.net/10468/3015
dc.description.abstract Fungal pathogen Candida albicans causes serious nosocomial infections in patients, in part, due to formation of drug-resistant biofilms. Protein kinases (PK) and transcription factors (TF) mediate signal transduction and transcription of proteins involved in biofilm development. To discover biofilm-related PKs, a collection of 63 C. albicans PK mutants was screened twice independently with microtiter plate-based biofilm assay (XTT). Thirty-eight (60%) mutants showed different degrees of biofilm impairment with the poor biofilm formers additionally possessing filamentation defects. Most of these genes were already known to encode proteins associated with Candida morphology and biofilms but VPS15, PKH3, PGA43, IME2 and CEX1, were firstly associated with both processes in this study. Previous studies of Holcombe et al. (2010) had shown that bacterial pathogen, Pseudomonas aeruginosa can impair C. albicans filamentation and biofilm development. To investigate their interaction, the good biofilm former PK mutants of C. albicans were assessed for their response to P. aeruginosa supernatants derived from two strains, wildtype PAO1 and homoserine lactone (HSL)-free mutant ΔQS, without finding any nonresponsive mutants. This suggested that none of the PKs in this study was implicated in Candida-Pseudomonas signaling. To screen promoter sequences for overrepresented TFs across C. albicans gene sets significantly up/downregulated in presence of bacterial supernatants from Holcombe et al. (2010) study, TFbsST database was created online. The TFbsST database integrates experimentally verified TFs of Candida to analyse promoter sequences for TF binding sites. In silico studies predicted that Efg1p was overrepresented in C. albicans and C. parapsilosis RBT family genes. en
dc.description.sponsorship Higher Education Authority (PRTLI5 Structured PhD programme) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher University College Cork en
dc.rights © 2016, Nina Konstantinidou. en
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/ en
dc.subject Candida albicans en
dc.subject Candida albicans morphology and biofilms en
dc.subject Pseudomonas aeruginosa en
dc.subject TFbsST database en
dc.subject In silico analysis of Candida albicans promoter sequences en
dc.subject Protein kinase en
dc.subject Biofilms en
dc.subject Filamentation en
dc.subject Transcription factor binding site motifs en
dc.subject Promoter regions en
dc.subject Yeast hyphae en
dc.subject Candida albicans morphology and interaction with Pseudomonas aeruginosa en
dc.title Interaction between Candida albicans and Pseudomonas aeruginosa en
dc.type Doctoral thesis en
dc.type.qualificationlevel Doctoral Degree (Structured) en
dc.type.qualificationname PhD (Science) en
dc.internal.availability Full text available en
dc.check.info No embargo required en
dc.description.version Accepted Version
dc.contributor.funder Higher Education Authority en
dc.description.status Not peer reviewed en
dc.internal.school Microbiology en
dc.check.type No Embargo Required
dc.check.reason No embargo required en
dc.check.opt-out Not applicable en
dc.thesis.opt-out false
dc.check.embargoformat Not applicable en
ucc.workflow.supervisor j.morrissey@ucc.ie
dc.internal.conferring Autumn 2016 en


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© 2016, Nina Konstantinidou. Except where otherwise noted, this item's license is described as © 2016, Nina Konstantinidou.
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