Transposon mutagenesis in bifidobacterium breve: construction and characterization of a Tn5 transposon mutant library for bifidobacterium breve UCC2003

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dc.contributor.author Ruiz, Lorena
dc.contributor.author O'Connell Motherway, Mary
dc.contributor.author Lanigan, Noreen
dc.contributor.author van Sinderen, Douwe
dc.date.accessioned 2016-08-31T14:20:55Z
dc.date.available 2016-08-31T14:20:55Z
dc.date.issued 2013-05-30
dc.identifier.citation Ruiz, L., O’Connell Motherway, M., Lanigan N. and van Sinderen D. (2013) 'Transposon mutagenesis in bifidobacterium breve: construction and characterization of a Tn5 transposon mutant library for bifidobacterium breve UCC2003', PLoS ONE 8(5): e64699. http://dx.doi.org/10.1371/journal.pone.0064699 en
dc.identifier.volume 8 en
dc.identifier.issued 5 en
dc.identifier.startpage e64699-1 en
dc.identifier.endpage e64699-10 en
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10468/3042
dc.identifier.doi 10.1371/journal.pone.0064699
dc.description.abstract Bifidobacteria are claimed to contribute positively to human health through a range of beneficial or probiotic activities, including amelioration of gastrointestinal and metabolic disorders, and therefore this particular group of gastrointestinal commensals has enjoyed increasing industrial and scientific attention in recent years. However, the molecular mechanisms underlying these probiotic mechanisms are still largely unknown, mainly due to the fact that molecular tools for bifidobacteria are rather poorly developed, with many strains lacking genetic accessibility. In this work, we describe the generation of transposon insertion mutants in two bifidobacterial strains, B. breve UCC2003 and B. breve NCFB2258. We also report the creation of the first transposon mutant library in a bifidobacterial strain, employing B. breve UCC2003 and a Tn5-based transposome strategy. The library was found to be composed of clones containing single transposon insertions which appear to be randomly distributed along the genome. The usefulness of the library to perform phenotypic screenings was confirmed through identification and analysis of mutants defective in D-galactose, D-lactose or pullulan utilization abilities. en
dc.description.sponsorship Science Foundation Ireland (Irish Government’s National Development Plan; SFI grants 02/CE/B124 and 07/CE/B1368); HRB postdoctoral fellowship (grant PDTM/20011/9) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher PLoS ONE en
dc.rights © 2013 Ruiz et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. en
dc.rights.uri http://creativecommons.org/licenses/by/2.5/ en
dc.subject Bifidobacterium breve en
dc.subject Transposon insertion mutants en
dc.subject Bifidobacteria en
dc.subject Gastrointestinal disorders en
dc.subject Metabolic disorders en
dc.title Transposon mutagenesis in bifidobacterium breve: construction and characterization of a Tn5 transposon mutant library for bifidobacterium breve UCC2003 en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Douwe van Sinderen, Department of Microbiology, University College Cork, National University of Ireland, Western Road, Cork, Ireland. Email: d.vansinderen@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Health Research Board en
dc.contributor.funder Science Foundation Ireland en
dc.description.status Peer reviewed en
dc.identifier.journaltitle PLoS One en
dc.internal.copyrightchecked !!CORA!! en


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© 2013 Ruiz et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Except where otherwise noted, this item's license is described as © 2013 Ruiz et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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