In vitro antioxidant and immunomodulatory activity of transglutaminase-treated sodium caseinate hydrolysates

Show simple item record

dc.contributor.author Cermeño, Maria
dc.contributor.author FitzGerald, Richard J.
dc.contributor.author O'Brien, Nora M.
dc.date.accessioned 2016-09-21T08:40:10Z
dc.date.available 2016-09-21T08:40:10Z
dc.date.issued 2016-08-31
dc.identifier.citation Cermeño, M., FitzGerald, R.J. and O’Brien, N.M. (2016) 'In vitro antioxidant and immunomodulatory activity of transglutaminase-treated sodium caseinate hydrolysates', International Dairy Journal, 63, pp. 107-114. doi: 10.1016/j.idairyj.2016.08.007 en
dc.identifier.volume 63 en
dc.identifier.startpage 107 en
dc.identifier.endpage 114 en
dc.identifier.issn 0958-6946
dc.identifier.uri http://hdl.handle.net/10468/3104
dc.description.abstract Sodium caseinate (NaCN) was incubated prior to and after hydrolysis with a microbial transglutaminase (TGase) and hydrolysed with Prolyve 1000. The resultant hydrolysates were tested for their immunomodulatory and antioxidant activity. TGase-treated hydrolysates significantly reduced (p < 0.05) the production of IL-6 at 0.5 and 1 mg mL−1 and the non-TGase treated hydrolysate reduced the production of IL-6 at 1 mg mL−1 in concanavalin (ConA) stimulated Jurkat T cells. None of the samples had an effect on IL-2. The hydrolysates showed higher oxygen radical absorbance capacity assay and ferric reducing antioxidant power activity than unhydrolysed NaCN, but no significant (p > 0.05) differences were found between the TGase-treated and non-TGase-treated samples. In the presence of hydrogen peroxide, the non-TGase-treated sample exhibited the highest DNA protective effect in U937 cells. These findings suggest that NaCN derived hydrolysates with and without treatment with TGase may exert specific antioxidant, genoprotective and anti-inflammatory effects. en
dc.description.sponsorship Department of Agriculture, Food and the Marine, Ireland (Food Institutional Research Measure (FIRM) Grant Number: 10 RD TMFRC 701) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Elsevier B.V. en
dc.rights © 2016, Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license. en
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ en
dc.subject TGase en
dc.subject Genoprotective en
dc.subject DNA en
dc.subject IL-6 cytokine en
dc.subject Anti-inflammatory en
dc.subject Fractionation en
dc.subject Isolation en
dc.subject Peptides en
dc.subject Bioactive hydrolysates en
dc.title In vitro antioxidant and immunomodulatory activity of transglutaminase-treated sodium caseinate hydrolysates en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Nora O'Brien, Food and Nutritional Sciences, University College Cork, Cork, Ireland. +353-21-490-3000 Email: nob@ucc.ie en
dc.internal.availability Full text available en
dc.check.info Access to this article is restricted until 12 months after publication by request of the publisher. en
dc.check.date 2017-08-31
dc.description.version Accepted Version en
dc.contributor.funder Department of Agriculture, Food and the Marine, Ireland
dc.description.status Peer reviewed en
dc.identifier.journaltitle International Dairy Journal en
dc.internal.IRISemailaddress nob@ucc.ie


Files in this item

This item appears in the following Collection(s)

Show simple item record

© 2016, Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license. Except where otherwise noted, this item's license is described as © 2016, Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license.
This website uses cookies. By using this website, you consent to the use of cookies in accordance with the UCC Privacy and Cookies Statement. For more information about cookies and how you can disable them, visit our Privacy and Cookies statement