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In vitro antioxidant and immunomodulatory activity of transglutaminase-treated sodium caseinate hydrolysates
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Accepted Version
Date
2016-08-31
Authors
Cermeño, Maria
FitzGerald, Richard J.
O'Brien, Nora M.
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier B.V.
Published Version
Abstract
Sodium caseinate (NaCN) was incubated prior to and after hydrolysis with a microbial transglutaminase (TGase) and hydrolysed with Prolyve 1000. The resultant hydrolysates were tested for their immunomodulatory and antioxidant activity. TGase-treated hydrolysates significantly reduced (p < 0.05) the production of IL-6 at 0.5 and 1 mg mL−1 and the non-TGase treated hydrolysate reduced the production of IL-6 at 1 mg mL−1 in concanavalin (ConA) stimulated Jurkat T cells. None of the samples had an effect on IL-2. The hydrolysates showed higher oxygen radical absorbance capacity assay and ferric reducing antioxidant power activity than unhydrolysed NaCN, but no significant (p > 0.05) differences were found between the TGase-treated and non-TGase-treated samples. In the presence of hydrogen peroxide, the non-TGase-treated sample exhibited the highest DNA protective effect in U937 cells. These findings suggest that NaCN derived hydrolysates with and without treatment with TGase may exert specific antioxidant, genoprotective and anti-inflammatory effects.
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Keywords
TGase , Genoprotective , DNA , IL-6 cytokine , Anti-inflammatory , Fractionation , Isolation , Peptides , Bioactive hydrolysates
Citation
Cermeño, M., FitzGerald, R.J. and O’Brien, N.M. (2016) 'In vitro antioxidant and immunomodulatory activity of transglutaminase-treated sodium caseinate hydrolysates', International Dairy Journal, 63, pp. 107-114. doi: 10.1016/j.idairyj.2016.08.007