Genome analysis and characterisation of the exopolysaccharide produced by Bifidobacterium longum subsp. longum 35624™

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dc.contributor.author Altmann, Friedrich
dc.contributor.author Kosma, Paul
dc.contributor.author O'Callaghan, Amy
dc.contributor.author Leahy, Sinead
dc.contributor.author Bottacini, Francesca
dc.contributor.author Molloy, Evelyn M.
dc.contributor.author Plattner, Stephan
dc.contributor.author Schiavi, Elisa
dc.contributor.author Gleinser, Marita
dc.contributor.author Groeger, David S.
dc.contributor.author Grant, Ray
dc.contributor.author Rodriguez Perez, Noelia
dc.contributor.author Healy, Selena
dc.contributor.author Svehla, Elisabeth
dc.contributor.author Windwarder, Markus
dc.contributor.author Hofinger, Andreas
dc.contributor.author O'Connell Motherway, Mary
dc.contributor.author Akdis, Cezmi A.
dc.contributor.author Xu, Jun
dc.contributor.author Roper, Jennifer
dc.contributor.author van Sinderen, Douwe
dc.contributor.author O'Mahony, Liam
dc.date.accessioned 2016-10-06T11:55:44Z
dc.date.available 2016-10-06T11:55:44Z
dc.date.issued 2016-09-22
dc.identifier.citation Altmann F., Kosma P., O’Callaghan A., Leahy S., Bottacini F. et al (2016) ‘Genome analysis and characterisation of the exopolysaccharide produced by Bifidobacterium longum subsp. longum 35624™,’ PLoS ONE 11(9): e0162983 (23pp). doi: 10.1371/journal.pone.0162983 en
dc.identifier.volume 11 en
dc.identifier.issued 9 en
dc.identifier.startpage e0162983-1 en
dc.identifier.endpage e0162983-23 en
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10468/3164
dc.identifier.doi 10.1371/journal.pone.0162983
dc.description.abstract The Bifibobacterium longum subsp. longum 35624™ strain (formerly named Bifidobacterium longum subsp. infantis) is a well described probiotic with clinical efficacy in Irritable Bowel Syndrome clinical trials and induces immunoregulatory effects in mice and in humans. This paper presents (a) the genome sequence of the organism allowing the assignment to its correct subspeciation longum; (b) a comparative genome assessment with other B. longum strains and (c) the molecular structure of the 35624 exopolysaccharide (EPS624). Comparative genome analysis of the 35624 strain with other B. longum strains determined that the sub-speciation of the strain is longum and revealed the presence of a 35624-specific gene cluster, predicted to encode the biosynthetic machinery for EPS624. Following isolation and acid treatment of the EPS, its chemical structure was determined using gas and liquid chromatography for sugar constituent and linkage analysis, electrospray and matrix assisted laser desorption ionization mass spectrometry for sequencing and NMR. The EPS consists of a branched hexasaccharide repeating unit containing two galactose and two glucose moieties, galacturonic acid and the unusual sugar 6-deoxy-L-talose. These data demonstrate that the B. longum 35624 strain has specific genetic features, one of which leads to the generation of a characteristic exopolysaccharide. en
dc.description.sponsorship European Commission (European Union Marie Curie training network, “TEAM-EPIC”); Swiss National Science Foundation (Grants project numbers CRSII3_154488 and 310030_144219); Christine Kühne Center for Allergy Research and Education; Science Foundation Ireland (SFI Grant No. SFI/12/RC/2273); Irish Research Council (Enterprise partnership scheme); Health Research Board (HRB postdoctoral fellowship (Grant No. PDTM/20011/9)); Alimentary Health Pharma Davos; Procter & Gamble, United States. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Public Library of Science en
dc.rights © 2016 Altmann et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.subject Exopolysaccharides en
dc.subject Bifidobacterium en
dc.subject Comparative genomics en
dc.subject Protons en
dc.subject Monosaccharides en
dc.subject NMR spectroscopy en
dc.subject Gene prediction en
dc.subject Polysaccharides en
dc.title Genome analysis and characterisation of the exopolysaccharide produced by Bifidobacterium longum subsp. longum 35624™ en
dc.type Article (peer-reviewed) en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Health Research Board en
dc.contributor.funder Irish Research Council for Science, Engineering and Technology en
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder European Commission en
dc.contributor.funder Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung en
dc.contributor.funder Alimentary Health Pharma Davos, Switzerland en
dc.contributor.funder Christine Kühne Center for Allergy Research and Education, Switzerland en
dc.contributor.funder Procter & Gamble, United States. en
dc.description.status Peer reviewed en
dc.identifier.journaltitle PLoS ONE en
dc.internal.copyrightchecked !!CORA!! en
dc.internal.IRISemailaddress liam.omahony@siaf.uzh.ch en


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© 2016 Altmann et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Except where otherwise noted, this item's license is described as © 2016 Altmann et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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