Ruthenibacterium lactatiformans gen. nov., sp.nov., an anaerobic, lactate-producing member of the family Ruminococcaceae isolated from human faeces

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dc.contributor.author Shkoporov, Andrei N.
dc.contributor.author Chaplin, Andrei V.
dc.contributor.author Shcherbakova, Victoria A.
dc.contributor.author Suzina, Natalia E.
dc.contributor.author Kafarskaia, Lyudmila I.
dc.contributor.author Bozhenko, Vladimir K.
dc.contributor.author Efimov, Boris A.
dc.date.accessioned 2016-10-28T13:38:31Z
dc.date.available 2016-10-28T13:38:31Z
dc.date.issued 2016-08-01
dc.identifier.citation Shkoporov, A. N., Chaplin, A. V., Shcherbakova, V. A., Suzina, N. E., Kafarskaia, L. I., Bozhenko, V. K. and Efimov, B. A. (2016) ‘Ruthenibacterium lactatiformans gen. nov., sp. nov., an anaerobic, lactate-producing member of the family Ruminococcaceae isolated from human faeces’, International Journal of Systematic and Evolutionary Microbiology, 66, pp. 3041-3049. doi: 10.1099/ijsem.0.001143 en
dc.identifier.volume 66 en
dc.identifier.startpage 3041 en
dc.identifier.endpage 3049 en
dc.identifier.issn 1466-5026
dc.identifier.uri http://hdl.handle.net/10468/3226
dc.identifier.doi 10.1099/ijsem.0.001143
dc.description.abstract Two novel strains of Gram-stain-negative, rod-shaped, obligately anaerobic, non-spore-forming, non-motile bacteria were isolated from the faeces of healthy human subjects. The strains, designated as 585-1T and 668, were characterized by mesophilic fermentative metabolism, production of d-lactic acid, succinic acid and acetic acid as end products of d-glucose fermentation, prevalence of C18 : 1 ω9, C18 : 1 ω9 aldehyde, C16 : 0 and C16 : 1 ω7c fatty acids, presence of glycine, glutamic acid, lysine, alanine and aspartic acid in the petidoglycan peptide moiety and lack of respiratory quinones. Whole genome sequencing revealed the DNA G+C content was 56.4–56.6 mol%. The complete 16S rRNA gene sequences of the two strains shared 91.7/91.6 % similarity with Anaerofilum pentosovorans FaeT, 91.3/91.2 % with Gemmiger formicilis ATCC 27749T and 88.9/88.8 % with Faecalibacterium prausnitzii ATCC 27768T. On the basis of chemotaxonomic and genomic properties it was concluded that the strains represent a novel species in a new genus within the family Ruminococcaceae , for which the name Ruthenibacterium lactatiformans gen. nov., sp. nov. is proposed. The type strain of Ruthenibacterium lactatiformans is 585-1T (=DSM 100348T=VKM B-2901T). en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Microbiology Society en
dc.rights © 2016 IUMS. This is the Accepted Paper version of the following article: Shkoporov et al. Int J Syst Evol Microbiol, 66:3041-3049. The Version of Record is available online at http://dx.doi.org/10.1099/ijsem.0.001143 en
dc.subject Human gut microbiota en
dc.subject Ruminococcaceae en
dc.subject Ruthenibacterium en
dc.subject Lactatiformans en
dc.title Ruthenibacterium lactatiformans gen. nov., sp.nov., an anaerobic, lactate-producing member of the family Ruminococcaceae isolated from human faeces en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother APC Microbiome Institute, University College Cork, Ireland, +353 21 490 3000 Email: andrey.shkoporov@ucc.ie en
dc.internal.availability Full text available en
dc.check.info Access to this article is restricted until 12 months after publication by the request of the publisher, en
dc.check.date 2017-08-01
dc.description.version Accepted Version en
dc.description.status Peer reviewed en
dc.identifier.journaltitle International Journal of Systematic and Evolutionary Microbiology en
dc.internal.copyrightchecked !!CORA!! en
dc.internal.IRISemailaddress andrey.shkoporov@ucc.ie en


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