Development of an antigen-driven colitis model to study presentation of antigens by antigen presenting cells to T cells

Show simple item record Rossini, Valerio Radulovic, Katarina Riedel, Christian U. Niess, Jan Hendrik 2016-11-08T11:57:20Z 2016-11-08T11:57:20Z 2016-09-18
dc.identifier.citation Rossini, V., Radulovic, K., Riedel, C.U. and Niess, J.H. (2016) ‘Development of an antigen-driven colitis model to study presentation of antigens by antigen presenting cells to T cells’, Journal of Visualized Experiments, (115), e54421 (11pp). doi:10.3791/54421 en
dc.identifier.issued 115 en
dc.identifier.startpage 1 en
dc.identifier.endpage 11 en
dc.identifier.issn 1940-087X
dc.identifier.doi 10.3791/54421
dc.description.abstract Inflammatory bowel disease (IBD) is a chronic inflammation which affects the gastrointestinal tract (GIT). One of the best ways to study the immunological mechanisms involved during the disease is the T cell transfer model of colitis. In this model, immunodeficient mice (RAG-/-recipients) are reconstituted with naive CD4+ T cells from healthy wild type hosts. This model allows examination of the earliest immunological events leading to disease and chronic inflammation, when the gut inflammation perpetuates but does not depend on a defined antigen. To study the potential role of antigen presenting cells (APCs) in the disease process, it is helpful to have an antigen-driven disease model, in which a defined commensal-derived antigen leads to colitis. An antigen driven-colitis model has hence been developed. In this model OT-II CD4+ T cells, that can recognize only specific epitopes in the OVA protein, are transferred into RAG-/- hosts challenged with CFP-OVA-expressing E. coli. This model allows the examination of interactions between APCs and T cells in the lamina propria. en
dc.description.sponsorship Swiss National Foundation (SNSF 310030_146290) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Journal of Visualized Experiments en
dc.rights © 2016, Journal of Visualized Experiments. en
dc.subject Immunology en
dc.subject CX3CR1+ phagocytes en
dc.subject CD4+ T cell en
dc.subject Colitis en
dc.subject Inflammatory bowel disease en
dc.subject Escherichia coli en
dc.subject Confocal microscopy en
dc.title Development of an antigen-driven colitis model to study presentation of antigens by antigen presenting cells to T cells en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Valerio Rossini, Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland. +353-21-490-3000 Email: valerio.rossini@ucc en
dc.internal.availability Full text available en Access to this article is restricted until 24 months after publication by request of the publisher. en 2018-09-18
dc.description.version Published Version en
dc.contributor.funder Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Journal of Visualized Experiments en
dc.internal.IRISemailaddress valerio.rossini@ucc
dc.identifier.articleid e54421

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