Occurrence of OsHV-1 in Crassostrea gigas cultured in Ireland during an exceptionally warm summer. Selection of less susceptible oysters

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dc.contributor.author Prado-Alvarez, Maria
dc.contributor.author Darmody, Grainne
dc.contributor.author Hutton, Stephen
dc.contributor.author O'Reilly, Amy
dc.contributor.author Lynch, Sharon A.
dc.contributor.author Culloty, Sarah C.
dc.date.accessioned 2016-11-16T15:47:21Z
dc.date.available 2016-11-16T15:47:21Z
dc.date.issued 2016-11-08
dc.identifier.citation Prado-Alvarez, M., G. Darmody, S. Hutton, A. O'Reilly, S. A. Lynch and S. C. Culloty (2016). ‘Occurrence of OsHV-1 in Crassostrea gigas cultured in Ireland during an exceptionally warm summer. Selection of less susceptible oysters’, Frontiers in Physiology 7, 492 (14pp). doi: 10.3389/fphys.2016.00492 en
dc.identifier.volume 7 en
dc.identifier.startpage 492-1 en
dc.identifier.endpage 492-14 en
dc.identifier.issn 1664-042X
dc.identifier.uri http://hdl.handle.net/10468/3277
dc.identifier.doi 10.3389/fphys.2016.00492
dc.description.abstract The occurrence of OsHV-1, a herpes virus causing mass mortality in the Pacific oyster Crassostrea gigas was investigated with the aim to select individuals with different susceptibility to the infection. Naïve spat transferred to infected areas and juveniles currently being grown at those sites were analyzed using molecular and histology approaches. The survey period distinguishes itself by very warm temperatures reaching up to 3.5°C above the average. The virus was not detected in the virus free area although a spread of the disease could be expected due to high temperatures. Overall mortality, prevalence of infection and viral load was higher in spat confirming the higher susceptibility in early life stages. OsHV-1 and oyster mortality were detected in naïve spat after 15 days of cohabitation with infected animals. Although, infection was associated with mortality in spat, the high seawater temperatures could also be the direct cause of mortality at the warmest site. One stock of juveniles suffered an event of abnormal mortality that was significantly associated with OsHV-1 infection. Those animals were infected with a previously undescribed microvariant whereas the other stocks were infected with OsHV-1 μVar. Cell lesions due to the infection were observed by histology and true infections were corroborated by in situ hybridization. Survivors from the natural outbreak were exposed to OsHV-1 μVar by intramuscular injection and were compared to naïve animals. The survival rate in previously exposed animals was significantly higher than in naïve oysters. Results derived from this study allowed the selection of animals that might possess interesting characteristics for future analysis on OsHV-1 resistance. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Frontiers Media en
dc.rights © 2016 Prado-Alvarez, Darmody, Hutton, O’Reilly, Lynch and Culloty. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.subject Crassostrea gigas en
dc.subject OsHV-1µvar en
dc.subject Prevalence en
dc.subject Resistance en
dc.subject qPCR en
dc.subject ISH en
dc.subject Pacific oyster en
dc.title Occurrence of OsHV-1 in Crassostrea gigas cultured in Ireland during an exceptionally warm summer. Selection of less susceptible oysters en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Maria Prado-Alvarez, Aquaculture & Fisheries Development Centre, University College Cork, T: +353 21 490 3000 E: m.pradoalvarez@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Seventh Framework Programme en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Frontiers in Physiology en
dc.internal.copyrightchecked !!CORA!! en
dc.internal.IRISemailaddress m.pradoalvarez@ucc.ie en
dc.relation.project info:eu-repo/grantAgreement/EC/FP7::SP3::PEOPLE/327932/EU/Herpes virus in Irish oysters and identification of resistant stocks/HERPISH en


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© 2016 Prado-Alvarez, Darmody, Hutton, O’Reilly, Lynch and Culloty. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Except where otherwise noted, this item's license is described as © 2016 Prado-Alvarez, Darmody, Hutton, O’Reilly, Lynch and Culloty. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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