Behavioural and neurochemical consequences of chronic gut microbiota depletion during adulthood in the rat

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dc.contributor.author Hoban, Alan E.
dc.contributor.author Moloney, Rachel D.
dc.contributor.author Golubeva, Anna V.
dc.contributor.author McVey Neufeld, Karen A.
dc.contributor.author O'Sullivan, Orla
dc.contributor.author Patterson, Elaine
dc.contributor.author Stanton, Catherine
dc.contributor.author Dinan, Timothy G.
dc.contributor.author Clarke, Gerard
dc.contributor.author Cryan, John F.
dc.date.accessioned 2016-11-18T11:25:48Z
dc.date.available 2016-11-18T11:25:48Z
dc.date.issued 2016-10-11
dc.identifier.citation Hoban, A. E., Moloney, R. D., Golubeva, A. V., McVey Neufeld, K. A., O’Sullivan, O., Patterson, E., Stanton, C., Dinan, T. G., Clarke, G. and Cryan, J. F. (2016) 'Behavioural and neurochemical consequences of chronic gut microbiota depletion during adulthood in the rat', Neuroscience, 339, pp. 463-477. doi: 10.1016/j.neuroscience.2016.10.003 en
dc.identifier.volume 339 en
dc.identifier.startpage 463 en
dc.identifier.endpage 477 en
dc.identifier.issn 0306-4522
dc.identifier.uri http://hdl.handle.net/10468/3290
dc.identifier.doi 10.1016/j.neuroscience.2016.10.003
dc.description.abstract Gut microbiota colonization is a key event for host physiology that occurs early in life. Disruption of this process leads to altered brain development which ultimately manifests as changes in brain function and behaviour in adulthood. Studies using germ-free mice highlight the extreme impact on brain health that results from life without commensal microbes, however the impact of microbiota disturbances occurring in adulthood is less studied. To this end, we depleted the gut microbiota of 10-week-old male Sprague Dawley rats via chronic antibiotic treatment. Following this marked, sustained depletion of the gut bacteria, we investigated behavioural and molecular hallmarks of gut-brain communication. Our results reveal that depletion of the gut microbiota during adulthood results in deficits in spatial memory as tested by Morris water maze, increased visceral sensitivity and a greater display of depressive-like behaviours in the forced swim test. In tandem with these clear behavioural alterations we found change in altered CNS serotonin concentration along with changes in the mRNA levels of corticotrophin releasing hormone receptor 1 and glucocorticoid receptor. Additionally, we found changes in the expression of BDNF, a hallmark of altered microbiota-gut-brain axis signaling. In summary, this model of antibiotic-induced depletion of the gut microbiota can be used for future studies interested in the impact of the gut microbiota on host health without the confounding developmental influence of early-life microbial alterations. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Elsevier Ltd. en
dc.relation.uri http://www.sciencedirect.com/science/article/pii/S0306452217300143
dc.relation.uri http://www.sciencedirect.com/science/article/pii/S0306452216305127
dc.rights © 2016, International Brain Research Organization. Published by Elsevier Ltd. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ en
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/ en
dc.subject Colonization en
dc.subject Brain development en
dc.subject Brain function en
dc.subject Behaviour en
dc.subject Antibiotic en
dc.subject Depletion en
dc.subject Gut bacteria en
dc.subject Gut microbiota en
dc.subject Gut-brain communication en
dc.subject Spatial memory en
dc.subject Visceral sensitivity en
dc.subject Serotonin en
dc.subject Cognition en
dc.subject Brain monoamines en
dc.subject Depressive-like behaviours en
dc.title Behavioural and neurochemical consequences of chronic gut microbiota depletion during adulthood in the rat en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother John Cryan, APC Microbiome Institute, University College Cork, Cork, Ireland. T: +353-21-490-3000 E: j.cryan@ucc.ie en
dc.internal.availability Full text available en
dc.check.info Access to this article is restricted until 12 months after publication by request of the publisher. en
dc.check.date 2017-10-11
dc.description.version Accepted Version en
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder Health Research Board en
dc.contributor.funder Department of Agriculture, Food and the Marine en
dc.description.status Peer reviewed en
dc.internal.IRISemailaddress j.cryan@ucc.ie
dc.internal.IRISemailaddress j.cryan@ucc.ie en
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/


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© 2016, International Brain Research Organization. Published by Elsevier Ltd. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ Except where otherwise noted, this item's license is described as © 2016, International Brain Research Organization. Published by Elsevier Ltd. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
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