Design, modelling and preliminary characterisation of microneedle-based electrodes for tissue electroporation in vivo

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Date
2016-11
Authors
O'Mahony, Conor
Houlihan, Ruth
Grygoryev, Konstantin
Ning, Zhenfei
Williams, John
Moore, Thomas F.
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IOP Publishing
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Abstract
We analysed the use of microneedle-based electrodes to enhance electroporation of mouse testis with DNA vectors for production of transgenic mice. Different microneedle formats were developed and tested, and we ultimately used electrodes based on arrays of 500 μm tall microneedles. In a series of experiments involving injection of a DNA vector expressing Green Fluorescent Protein (GFP) and electroporation using microneedle electrodes and a commercially available voltage supply, we compared the performance of flat and microneedle electrodes by measuring GFP expression at various timepoints after electroporation. Our main finding, supported by both experimental and simulated data, is that needles significantly enhanced electroporation of testis.
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Keywords
Microneedle formats , Microneedle electrodes , GFP expression , Electroporation
Citation
O'Mahony, C., Houlihan, R., Grygoryev, K., Ning, Z., Williams, J. and Moore, T. (2016) 'Design, modelling and preliminary characterisation of microneedle-based electrodes for tissue electroporation in vivo'. Journal of Physics: Conference Series, 757 :012040-1-012040-6. doi: 10.1088/1742-6596/757/1/012040